Abstract Patulin is a type of mycotoxin that can compromise both food quality and human health, and its potential toxic effects on human health are concerning. Therefore, strict monitoring of patulin content in food systems is necessary. However, discrimination and quantification of this toxin are difficult because of its extremely low concentration at pg/g levels in food samples. A highly specific sample preparation method is, thus, vital for patulin analysis. Herein, we present an innovative approach for the facile and controllable preparation of an aptamer-functionalized capillary monolithic polymer hybrid for the determination of patulin in food samples with high specificity and high affinity by using the aptamer-functionalized monolithic capillary polymer hybrid as a sorbent for solid-phase microextraction (SPME) coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The results confirmed that the developed SPME-UHPLC-MS/MS method was a sensitive and selective because of a low detection limit of 2.17 pmol/L and a linear range extending from 0.00811 to 8.11 nmol/L. Patulin was found to be present in apple juice samples (4.34 ng/kg). Furthermore, the recoveries were 85.4–106% with relative standard deviations (n = 5) of 4.1–7.3%. Thus, the highly selective method will facilitate the ultrasensitive detection of target molecules in diverse applications.

中文翻译：

---

用于高选择性和高效识别展青霉素的适体功能化毛细管整体柱的开发

摘要 展青霉素是一种真菌毒素，会危害食品质量和人类健康，其对人类健康的潜在毒性影响令人担忧。因此，有必要对食品系统中的展青霉素含量进行严格监测。然而，这种毒素在食品样品中的 pg/g 浓度极低，因此很难对其进行区分和定量。因此，高度特异性的样品制备方法对于展青霉素分析至关重要。在此处，我们提出了一种创新的方法，通过使用适体功能化的整体毛细管聚合物杂化物作为固体吸附剂，以高特异性和高亲和力测定食品样品中的展青霉素，以方便和可控地制备适体功能化的毛细管整体聚合物杂化物。相微萃取 (SPME) 结合超高效液相色谱-串联质谱 (UHPLC-MS/MS)。结果证实，由于 2.17 pmol/L 的低检测限和 0.00811 至 8.11 nmol/L 的线性范围，开发的 SPME-UHPLC-MS/MS 方法具有灵敏度和选择性。苹果汁样品中发现棒曲霉素 (4.34 ng/kg)。此外，回收率为 85.4-106%，相对标准偏差 (n = 5) 为 4.1-7.3%。因此，