Moringa oleifera Lam. (M. oleifera) leaves have long been consumed as both nutritive vegetable and popular folk medicine for hyperglycemia and hyperlipidemia in Kenya communities. In the current study, in vitro inhibition by M. oleifera leaf extract (MOLE, 90% (v/v) ethanol) of α-glucosidase and pancreatic lipase was demonstrated, followed by determination of the effects of MOLE on both glucose consumption and lipid levels (TC, TG, HDL-C and LDL-C) in 3T3-L1 cells. Potential ligands in MOLE were fast screened using affinity ultrafiltration LC-MS, and 14 and 10 components displayed certain binding affinity to α-glucosidase and pancreatic lipase, respectively. Docking studies revealed the binding energies and hydrogen bonds between potential ligands and enzymes. This study suggests that M. oleifera leaves may be a promising natural source for the prevention and treatment of hyperglycemia and hyperlipidemia as well as a functional food or other product for health care in the near future.

辣木林。（M. oleifera）叶子长期以来被用作营养蔬菜和流行的民间药物，以治疗肯尼亚社区的高血糖和高血脂症。在当前的研究中，油橄榄分枝杆菌的体外抑制证明了叶提取物（MOLE，90％（v / v）乙醇）的α-葡萄糖苷酶和胰腺脂肪酶，然后测定了MOLE对葡萄糖消耗和脂质水平（TC，TG，HDL-C和LDL- C）在3T3-L1细胞中。使用亲和超滤LC-MS快速筛选MOLE中的潜在配体，并且14和10个组分分别显示出对α-葡萄糖苷酶和胰腺脂肪酶的一定结合亲和力。对接研究表明潜在配体和酶之间的结合能和氢键。这项研究表明，油橄榄叶可能是预防和治疗高血糖和高血脂症的有前途的天然来源，并且在不久的将来可能是功能性食品或其他保健产品。