In this study, a new method for simultaneous determination of cyantraniliprole, chlorantraniliprole, tetrachlorantraniliprole, cyclaniliprole and flubendiamide in edible mushrooms by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) combined with a modified QuEChERS procedure. The samples were extracted using acetonitrile and then cleaned up by primary secondary amine (PSA) and octadecylsilane (C18). The determination of these insecticides was achieved in less than 5 min using an electrospray ionization source in positive mode (ESI+) for cyantraniliprole and chlorantraniliprole, while negative mode (ESI−) for tetrachlorantraniliprole, cyclaniliprole and flubendiamide. The linearities of the calibrations for all target compounds were acceptable (R² ≥ 0.9922). The limits of detection and quantification were 0.05–2 μg kg⁻¹ and 5 μg kg⁻¹, respectively. Acceptable recoveries (73.5–110.2%) were acquired for these insecticides with RSDs less than 12.7%. The results demonstrated that the proposed method was effective and convenient for the determination of these insecticides in edible mushrooms.

在这项研究中，采用高效液相色谱-串联质谱（HPLC-MS / MS）结合改进的QuEChERS程序，同时测定食用菌中的氰基苯丙胺，氯吡喃，四氯苯丙胺，环苯丙胺和氟苯二酰胺的新方法。样品用乙腈萃取，然后用伯仲胺（PSA）和十八烷基硅烷（C18）净化。使用电喷雾电离源在不到5分钟的时间内就可以确定这些杀虫剂，其中氰基氰基丙烯腈和氯氰基丙烯腈为正模式（ESI +），而四氯蒽基腈，环氰基丙烯腈和氟苯二酰胺为负模式（ESI-）。所有目标化合物的校准线性均可接受（R ² ≥0.9922）。检测和定量的极限分别为0.05–2μgkg ^-1和5μgkg ^-1。这些杀虫剂的RSD小于12.7％的回收率是可接受的（73.5-110.2％）。结果表明，该方法对食用菌中这些杀虫剂的测定是有效，简便的。