The influence of electrostatic field on the direct interspecies electron transfer (DIET) pathways for methane production was investigated in a batch bioelectrochemical anaerobic digester (BEAD). The ultimate methane production and methane yield in the BEAD reactor saturated to 925±29 mL/L and 309.9±9.6 mL CH₄/g COD, respectively, which were much higher than 616±3 mL/L and 205.4±205.4 mL CH₄/g COD in the anaerobic digester (AD). In the cyclic voltammogram (CV) for bulk solution, the oxidation peak current was 0.52 mA in the BEAD reactor, which was higher than 0.24 mA of AD reactor. This shows that the oxidizing ability of microorganisms was greatly improved in the BEAD reactor. Anaerolineaceae, a well-known electroactive bacterial family, was well enriched in the BEAD reactor. It indicates that the electrostatic field can enrich the electroactive bacteria and activate the DIET pathways for methane production. Moreover, the conductive material (activated carbon) further improved the performance of BEAD reactor, implies that the conductivities of bulk solution is one of the important parameters for the DIET pathways.

在间歇式生物电化学厌氧消化器（BEAD）中研究了静电场对甲烷直接产生的种间电子转移（DIET）途径的影响。BEAD反应器中的最终甲烷产量和甲烷产率分别达到925±29 mL / L和309.9±9.6 mL CH ₄ / g COD，远远高于616±3 mL / L和205.4±205.4 mL CH _4。/ g厌氧消化器（AD）中的COD。在本体溶液的循环伏安图（CV）中，BEAD反应器中的氧化峰电流为0.52 mA，高于AD反应器中的0.24 mA。这表明在BEAD反应器中微生物的氧化能力大大提高。厌氧菌科，一个著名的电活性细菌家族，在BEAD反应器中富集了很多。这表明静电场可以富集电活性细菌并激活DIET生成甲烷的途径。此外，导电材料（活性炭）进一步提高了BEAD反应器的性能，表明本体溶液的电导率是DIET途径的重要参数之一。