Introduction

Citrus hystrix is widely used by Indonesians as a traditional medicine for gastrointestinal diseases, including Salmonella spp. infection. We investigated the antibacterial activity of the ethanolic peel extract of C. hystrix against Salmonella typhimurium.

Methods

The antibacterial activity was evaluated both in vitro and in vivo. The minimum inhibitory concentration (MIC) of the extract was determined at a concentration of 0.625% by agar dilution assay. Later, the in vivo antibacterial activity was examined by the administration of 16 mg of the extract daily for three consecutive days in a mouse model infected with S. typhimurium.

Results

The bacterial loads of S. typhimurium in the ileum, liver, and spleen decreased after 24 h of administration of the extract (p = 0.00008, p = 0.00084, and p = 0.00003, respectively).

Conclusion

The ethanolic peel extract of C. hystrix shows antibacterial activity against S. typhimurium, indicating the potential of C. hystrix as an effective treatment for Salmonella spp. infection.

中文翻译：

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Citrus hystrix 对沙门氏菌的抗菌活性。感染

介绍

Citrus hystrix被印度尼西亚人广泛用作治疗胃肠道疾病的传统药物，包括沙门氏菌属。感染。我们研究了C. hystrix的乙醇果皮提取物对鼠伤寒沙门氏菌的抗菌活性。

方法

体外和体内评价抗菌活性。通过琼脂稀释法测定提取物的最低抑菌浓度 (MIC) 为 0.625%。后来，通过在 感染鼠伤寒沙门氏菌的小鼠模型中连续三天每天服用 16毫克提取物来检查体内抗菌活性。

结果

施用提取物24小时后，回肠、肝脏和脾脏中鼠伤寒沙门氏菌的细菌负荷降低 （分别为p  =  0.00008、p  =  0.00084 和p  =  0.00003）。

结论

C. hystrix的乙醇果皮提取物显示出对S. typhimurium 的抗菌活性，表明C. hystrix作为沙门氏菌属有效治疗的潜力。感染。