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Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia.
Diagnostic Microbiology and Infectious Disease ( IF 2.1 ) Pub Date : 2020-07-05 , DOI: 10.1016/j.diagmicrobio.2020.115125 Salma Abid 1 , Sana Ferjani 2 , Awatef El Moussi 1 , Asma Ferjani 1 , Mejda Nasr 3 , Ichrak Landolsi 3 , Karima Saidi 3 , Hanène Gharbi 3 , Hajer Letaief 4 , Aicha Hechaichi 4 , Mouna Safer 4 , Nissaf Bouafif Ep Ben Alaya 4 , Ilhem Boutiba-Ben Boubaker 1
中文翻译：
对突尼斯 SARS-CoV-2 分子检测样本池进行评估，以筛选无症状人员。
更新日期：2020-08-05
Diagnostic Microbiology and Infectious Disease ( IF 2.1 ) Pub Date : 2020-07-05 , DOI: 10.1016/j.diagmicrobio.2020.115125 Salma Abid 1 , Sana Ferjani 2 , Awatef El Moussi 1 , Asma Ferjani 1 , Mejda Nasr 3 , Ichrak Landolsi 3 , Karima Saidi 3 , Hanène Gharbi 3 , Hajer Letaief 4 , Aicha Hechaichi 4 , Mouna Safer 4 , Nissaf Bouafif Ep Ben Alaya 4 , Ilhem Boutiba-Ben Boubaker 1
Affiliation
The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10. Each laboratory should conduct its own evaluation and validation of pooling protocols according to its specific context.
中文翻译：
对突尼斯 SARS-CoV-2 分子检测样本池进行评估，以筛选无症状人员。
本研究的目的是测试 RT-PCR 测试的汇集方法，以检测 SARS-CoV-2 的低病毒载量。我们发现，在最多 10 个样本的池中仍然可以检测到单个阳性样本。每个实验室应根据其具体情况对池协议进行自己的评估和验证。
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