Oral administration of sucralose has been reported to stimulate food intake through inducing hypothalamic neuropeptide Y (NPY) in mice and fruit flies. However, the underlying mechanisms of action of sucralose in hypothermia and NPY and monoamine regulation remain unknown. The aim of the present study was to investigate central effects of sucralose on body temperature, NPY, and monoamine regulation, as well as its peripheral effects, in chicks. In Experiment 1, 5-day-old chicks were centrally injected with 1 μmol of sucralose, other sweeteners (erythritol and glucose), or saline. In Experiment 2, chicks were centrally injected with 0.2, 0.4, and 1.6 μmol of sucralose or saline. In Experiment 3, chicks were centrally injected with 0.8 μmol of sucralose or saline, with a co-injection of 100 μg fusaric acid (FA), an inhibitor of dopamine-β-hydroxylase, to examine the role dopamine in sucralose induced hypothermia. In Experiment 4, 7–16-day-old chicks were orally administered with 75, 150, and 300 mg/2 ml distilled water or sucralose, daily. We observed that the central injection of sucralose, but not other sweeteners, decreased body temperature (P < .05) in chicks; however, the oral injection did not influence body temperature, food intake, and body weight gain. Central sucralose administration decreased dopamine and serotonin and stimulated dopamine turnover rate in the hypothalamus significantly (P < .05). Notably, sucralose co-injection with FA impeded sucralose-induced hypothermia. Sucralose decreases body temperature potentially via central monoaminergic pathways in the hypothalamus.

据报道，口服三氯蔗糖可通过诱导小鼠和果蝇的下丘脑神经肽Y（NPY）刺激食物摄入。然而，三氯蔗糖在体温过低，NPY和单胺调节中的基本作用机制仍然未知。本研究的目的是研究三氯蔗糖对雏鸡体温，NPY和单胺调节的主要作用及其周边作用。在实验1中，向5天大的雏鸡集中注射1μmol三氯蔗糖，其他甜味剂（赤藓糖醇和葡萄糖）或盐水。在实验2中，向小鸡集中注射0.2、0.4和1.6μmol三氯蔗糖或盐水。在实验3中，向小鸡集中注射0.8μmol三氯蔗糖或生理盐水，并同时注射100μg富沙酸（FA），多巴胺-β-羟化酶的抑制剂，以检查多巴胺在三氯蔗糖诱导的体温过低中的作用。在实验4中，每天给7–16日龄的小鸡口服75、150和300 mg / 2 ml蒸馏水或三氯蔗糖。我们观察到，三氯蔗糖的集中注射而不是其他甜味剂的注射降低了体温（P  <.05）。但是，口服注射并不会影响体温，食物摄入和体重增加。中央三氯蔗糖给药可显着降低下丘脑中的多巴胺和5-羟色胺，并刺激多巴胺周转率（P  <.05）。值得注意的是，三氟蔗糖与FA的共同注射阻碍了三氯蔗糖引起的体温过低。三氯蔗糖可能通过下丘脑中枢单胺能途径降低体温。