In the present work, direct electron transfer (DET) based biosensing system for the determination of glucose has been fabricated by utilizing gold binding peptide (GBP) fused flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) from Burkholderia cepacia. The GBP fused FAD-GDH was immobilized on the working electrode surface of screen-printed electrode (SPE) which consists of gold working electrode, a silver pseudo-reference electrode and a platinum counter electrode, to develop the biosensing system with compact design and favorable sensing ability. The bioelectrochemical and mechanical properties of GBP fused FAD-GDH (GDH-GBP) immobilized SPE (GDH-GBP/Au) were investigated. Here, the binding affinity of GDH-GBP on Au surface, was highly increased after fusion of gold binding peptide and its uniform monolayer was formed on Au surface. In the cyclic voltammetry (CV), GDH-GBP/Au displayed significantly high oxidative peak currents corresponding to glucose oxidation which is almost c.a. 10-fold enhanced value compared with that from native GDH immobilized SPE (GDH/Au). As well, GDH-GBP/Au has shown 92.37% of current retention after successive potential scans. In the chronoamperometry, its steady-state catalytic current was monitored in various conditions. The dynamic range of GDH-GBP/Au was shown to be 3-30 mM at 30 °C and exhibits high selectivity toward glucose in whole human blood. Additionally, temperature dependency of GDH-GBP/Au on DET capability was also investigated at 30-70 °C. Considering this efficient and stable glucose sensing with simple and easy sensor fabrication, GDH-GBP based sensing platform can provide new insight for future biosensor in research fields that rely on DET.

在当前的工作中，通过利用洋葱伯克霍尔德氏菌的金结合肽（GBP）融合的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FAD-GDH）制备了基于直接电子传输（DET）的葡萄糖测定生物传感系统。。将GBP融合的FAD-GDH固定在由金工作电极，银伪参比电极和铂对电极组成的丝网印刷电极（SPE）的工作电极表面上，以开发设计紧凑且性能优良的生物传感系统。感知能力。研究了GBP融合的FAD-GDH（GDH-GBP）固定化SPE（GDH-GBP / Au）的生物电化学和力学性能。在此，在金结合肽融合之后，GDH-GBP在Au表面上的结合亲和力大大增加，并且其均匀的单层在Au表面上形成。在循环伏安法（CV）中，GDH-GBP / Au显示出明显高的氧化峰电流，与葡萄糖氧化相对应，与天然GDH固定的SPE（GDH / Au）相比，该值几乎提高了约10倍。还有 连续电位扫描后，GDH-GBP / Au显示出92.37％的电流保持率。在计时电流法中，在各种条件下监测其稳态催化电流。GDH-GBP / Au的动态范围在30°C下显示为3-30 mM，对全血中的葡萄糖具有很高的选择性。另外，还在30-70°C下研究了GDH-GBP / Au对DET能力的温度依赖性。考虑到这种高效稳定的葡萄糖感测和简单易用的传感器制造，基于GDH-GBP的感测平台可以为依赖于DET的研究领域中的未来生物传感器提供新的见解。GDH-GBP / Au的动态范围在30°C下显示为3-30 mM，对全血中的葡萄糖具有很高的选择性。另外，还在30-70°C下研究了GDH-GBP / Au对DET能力的温度依赖性。考虑到这种高效稳定的葡萄糖感测和简单易用的传感器制造，基于GDH-GBP的感测平台可以为依赖于DET的研究领域中的未来生物传感器提供新的见解。GDH-GBP / Au的动态范围在30°C下显示为3-30 mM，对全血中的葡萄糖具有很高的选择性。另外，还在30-70°C下研究了GDH-GBP / Au对DET能力的温度依赖性。考虑到这种高效稳定的葡萄糖感测以及简单易用的传感器制造，基于GDH-GBP的感测平台可以为依赖于DET的研究领域中的未来生物传感器提供新的见解。