This paper reports on a comparison between nano-ZnO/CuO and nano-ZnO nitrocellulose membrane biosensors, both of which were fabricated using a simple and inexpensive sonication technique. To produce the nano-ZnO/CuO membranes, the technique involved sonication of 1% (w/v) ZnO and 1% (w/v) CuO nano-crystal colloidal suspensions, with a volume ratio of 1:2. The membranes were analysed by scanning electron microscopy and energy-dispersive spectroscopy, which showed the gradated distribution of nanoparticles in the membrane. Impedance spectroscopy demonstrated that the sonication resulted in a greater than two-fold enhancement of the output signal. Changes in impedance phase values, at a frequency of 100 Hz, were used to establish dose dependent responses for C-reactive protein (CRP). Limits of detection of 27 pg/mL for the 1% (w/v) nano-ZnO and 16 pg/mL for the 1% (w/v) nano-ZnO/CuO nitrocellulose membrane biosensors were demonstrated.

本文报道了纳米ZnO / CuO和纳米ZnO硝酸纤维素膜生物传感器之间的比较，这两种传感器都是使用简单且廉价的超声技术制造的。为了生产纳米ZnO / CuO膜，该技术涉及超声处理1％（w / v）ZnO和1％（w / v）CuO纳米晶体胶体悬浮液，体积比为1：2。通过扫描电子显微镜和能量分散光谱法分析膜，其显示了纳米颗粒在膜中的梯度分布。阻抗谱表明，超声处理使输出信号增强了两倍以上。阻抗相位值的变化（频率为100 Hz）用于建立C反应蛋白（CRP）的剂量依赖性响应。