Abstract Shrimp farming has been severely affected by Vibrio infections in southeastern Asia, with severe economic impacts. Invasive seaweeds are an increasing threat that can be strategically valorized by biotechnology, promoting economic development while contributing to ecologic remediation. Asparagopsis armata and Sargassum muticum are two such species that have been frequently reported as excellent bioactive compounds producers, namely anti-bacterial and antioxidant. Thus, A. armata and S. muticum were extracted in an industry-focused approach, using low amounts of ethanol and short extraction times at room temperature. Yield, in vitro antioxidant capacity by the 2,2-diphenyl−1-picrylhydrazyl radical scavenging assay, and in vitro antibacterial activity against Vibrio parahaemolyticus (microdilution) were optimized using Response Surface Methodology (RSM). Optimal extracts (one from each seaweed species) were incorporated in feed, and an in vivo trial using the whiteleg shrimp Penaeus vannamei was performed in industrial facilities using the supplemented feed (1.5, 3.5 and 7.5 g extract per kg feed). Feed quality (along an 8-week period under regular storage conditions) and shrimp performance (40-day trial) were studied, as well as post-challenge (infection by virulent Vibrio parahaemolyticus) mortality rate and hepatopancreas condition. The results from the RSM showed that lower extraction times and ethanol volumes maximized extracts' bioactive properties (A. armata – 20 min, 10 mL solvent per gram seaweed; S. muticum – 100 min, 10 mL.g−1). The seaweed extracts did not impact shrimp performance (no statistically significant differences in weight gain, feed conversion rate or survival). Asparagopsis armata selected extract at 7.5 g per kg feed was able to decrease feed contamination by fungi along time and reduce shrimp mortality by up to 50% upon challenge with V. parahaemolyticus. Despite lacking statistical significance, a difference in hepatopancreas condition after challenge could be identified in shrimp fed the supplemented feed.

中文翻译：

---

通过包含工业友好的海藻提取物来定制虾水产饲料以应对急性肝胰腺坏死病

摘要 在东南亚，对虾养殖受到弧菌感染的严重影响，对经济造成严重影响。入侵海藻是一种日益严重的威胁，可以通过生物技术进行战略性评估，促进经济发展，同时有助于生态修复。Asparagopsis armata 和 Sargassum muticum 是两个这样的物种，它们经常被报道为优秀的生物活性化合物生产者，即抗菌和抗氧化剂。因此，A. armata 和 S. muticum 是以行业为重点的方法提取的，在室温下使用少量乙醇和较短的提取时间。产量，体外抗氧化能力通过 2,2-二苯基-1-苦基肼自由基清除试验，使用响应面方法 (RSM) 优化对副溶血性弧菌（微量稀释）的体外抗菌活性。将最佳提取物（来自每种海藻的一种）加入饲料中，并在工业设施中使用补充饲料（每公斤饲料 1.5、3.5 和 7.5 克提取物）使用南美白对虾进行体内试验。研究了饲料质量（在常规储存条件下持续 8 周）和虾的性能（40 天试验），以及攻击后（毒性副溶血性弧菌感染）死亡率和肝胰腺状况。RSM 的结果表明，较低的提取时间和乙醇体积可最大限度地提高提取物的生物活性特性（A. armata – 20 分钟，每克海藻需要 10 mL 溶剂；S. muticum – 100 分钟，10 mL.g-1）。海藻提取物不影响虾的性能（在增重、饲料转化率或存活率方面没有统计学上的显着差异）。Asparagopsis armata 选择的提取物 7.5 g/kg 饲料能够随着时间的推移减少真菌对饲料的污染，并在受到副溶血性弧菌攻击时将虾死亡率降低 50%。尽管缺乏统计显着性，但可以在喂食补充饲料的虾中确定攻击后肝胰腺状况的差异。