Ensets (Ensete spp., family Musaceae), are multipurpose plants with great versatility and potential as a food security crop, although they are cultivated only in Ethiopia and few other regions of Africa. Currently due to loss of habitat, intensification of agriculture, genetic diversity of both wild and domestic ensets is at high risk of erosion. Ensete glaucum (Roxb.) Cheesman, a less-known and under-utilized crop wild relative of banana, is found as natural strands in North-eastern regions of India. No report exists on seed conservation or cryopreservation of E. glaucum and the present work was undertaken to fill this knowledge gap. Mature fruits of two seeded accessions of E. glaucum (MZU-DBT-T15 and HSB-8) were collected from plants in natural populations from two geographically distinct North-eastern region of India. Seed morphology, moisture content (MC) of seed and embryo, viability and germination of excised embryos, desiccation and cryosensitivity were studied. Fresh seed (31% MC) viability measured through in vitro germination of excised embryos was not significantly different in both accessions (87–89%). Seeds could tolerate desiccation up to 5–10% MC without significant loss in germination potential (83–84%) and safely stored up to 1 year at room temperature (RT), without loss of viability. Air dehydrated seeds (10 ± 2% MC) and embryos (12 ± 2% MC) were cryopreserved (in liquid and vapour phase of liquid nitrogen). High post-thaw recovery was achieved in both tissues i.e. seeds (80.2 ± 2.2%) and embryos (83.1 ± 2.6%) by in vitro culture of excised embryos on MS medium + 2 µM gibberellic acid + 1 µM 6-benzyl amino purine (BAP) + 1 µM ascorbic acid. The seedlings converted into multiple shoots (15–20 shoots/explant) within 2 months of culture on medium with MS salts + 10 µM BAP + 1 µM indole-3-acetic acid (IAA) + 1 µM ascorbic acid. This study is the first successful report of cryopreservation of E. glaucum using simple air dehydration. Using the method devised, seeds and zygotic embryos of the two accessions of E. glaucum are safely conserved in the Cryogenebank, while in vitro plantlets are maintained in the In Vitro Genebank of ICAR-NBPGR, New Delhi, India.

Ensets（Ensete spp。，Musaceae家族）是多功能植物，用途广泛，具有作为粮食安全作物的潜力，尽管它们仅在埃塞俄比亚和非洲其他少数地区种植。当前，由于栖息地的丧失，农业的集约化，野生和家畜的遗传多样性都处于遭受侵蚀的高风险中。Ensete glaucum（Roxb。）Cheesman是一种鲜为人知的，未被充分利用的农作物香蕉近缘种，在印度东北部地区被发现为天然链。目前尚无关于青冈肠杆菌的种子保存或冷冻保存的报告，目前正在进行的工作是为了填补这一知识空白。两个青豌豆种子的成熟果实（MZU-DBT-T15和HSB-8）是从印度东北两个地理上不同的自然种群中的植物中收集的。研究了种子的形态，种子和胚芽的含水量，被切胚的活力和萌发，干燥和低温敏感性。通过切除胚的体外发芽测得的新鲜种子（MC的活力为31％）在两种材料中均无显着差异（87-89％）。种子可以耐受高达5-10％的MC脱水，而不会显着降低发芽势（83-84％），并且可以在室温（RT）下安全保存长达1年，而不会丧失生存能力。将空气脱水的种子（10±2％MC）和胚胎（12±2％MC）冷冻保存（在液氮的液相和气相中）。种子（80.2±2.2％）和胚胎（83.1±2。6％）通过在MS培养基+ 2 µM赤霉素+ 1 µM 6-苄基氨基嘌呤（BAP）+ 1 µM抗坏血酸上体外培养切下的胚胎。在含有MS盐+ 10 µM BAP + 1 µM吲哚-3-乙酸（IAA）+ 1 µM抗坏血酸的培养基上培养的2个月内，幼苗转化为多枝芽（15-20芽/植株）。这项研究是冷冻保存的第一个成功报告。E.青草使用简单的空气脱水。使用该方法设计，种子和两个种质的合子胚E.灰绿在Cryogenebank安全地保守的，而在体外小植株保持在ICAR-NBPGR，新德里，印度的体外基因库。