Nanotechnology has provided an unprecedented opportunity in addressing challenges faced by cancer treatment. Nanomaterials represent a promising and versatile method in biomedical applications. PEGylation of nanomaterials is considered as the most effective strategy for commercial relevance. The purpose of this study was to develop a novel solvothermal synthesis of PEGylated tungsten trioxide (PEG-WO₃) and Ag-doped PEGylated tungsten trioxide (Ag/PEG-WO₃) (1:2 ratio) sheet-like nanocomposites and to investigate the in vitro anticancer activities of PEGylated WO₃ and Ag-doped PEGylated tungsten trioxide (Ag/PEG-WO₃) (1:2 ratio) nanoparticles on MCF-7 human breast cancer cells. The structural, morphological and textural properties were characterized by powder X-ray diffractometer, transmission electron microscope, UV-vis diffuse reflectance spectroscopy and FTIR. The XRD analysis proved that doping of Ag NPs into the lattice of PEGylated WO₃ and TEM image indicated the successful anchoring of spherical-like shape of Ag NPs approximately 6–8 nm in size onto the sheet-like PEGylated WO₃. Morphological changes, ATP proliferation, cytotoxicity, caspase activity and nuclear damage of NPs treated an MCF-7 cell line were assessed. The Ag-doped PEGylated WO₃ nanoparticles (10 μg/mL) showed more toxicity towards MCF-7 cells in LDH assay (p < 0.05), reduced the proliferation (p < 0.01) and induced the caspase 3/7 activities significantly (p < 0.01). The morphological changes of MCF-7 cells after the treatments and results of Hoechst stain indicated nuclear damage. These results indicate a successful synthesis of a novel solvothermal nanocomposite capable of inducing cancer cell death.

This graph shows the synthesis of PEG-Ag-doped WO₃ and its application on breast cancer cells.

纳米技术为应对癌症治疗所面临的挑战提供了前所未有的机会。纳米材料代表了生物医学应用中一种有前途且用途广泛的方法。纳米材料的聚乙二醇化被认为是实现商业相关性的最有效策略。这项研究的目的是开发一种新型的溶剂热合成聚乙二醇化三氧化钨（PEG-WO ₃）和掺银的聚乙二醇化三氧化钨（Ag / PEG-WO ₃）（1：2）片状纳米复合材料，并进行研究聚乙二醇化WO ₃和掺银聚乙二醇化三氧化钨（Ag / PEG-WO _3）的体外抗癌活性（1：2的比例）MCF-7人乳腺癌细胞上的纳米颗粒。用粉末X射线衍射仪，透射电子显微镜，紫外可见漫反射光谱和傅立叶变换红外光谱（FTIR）对结构，形貌和织构性质进行表征。X射线衍射分析表明，将Ag NPs掺入PEG化WO ₃的晶格和TEM图像表明，大小约为6-8 nm的球形Ag NPs成功地锚定在片状PEG化WO _3上。评估了MCF-7细胞系的NPs的形态学变化，ATP增殖，细胞毒性，半胱天冬酶活性和核损伤。掺Ag的PEG化WO ₃纳米颗粒（10μg/ mL）在LDH分析中对MCF-7细胞表现出更大的毒性（p <0.05），降低了增殖（p  <0.01），并显着诱导了caspase 3/7活性（p  <0.01）。处理后MCF-7细胞的形态变化和Hoechst染色结果表明细胞核损伤。这些结果表明能够诱导癌细胞死亡的新型溶剂热纳米复合材料的成功合成。

该图显示了PEG-Ag掺杂的WO ₃的合成及其在乳腺癌细胞上的应用。