A sequential combination of laccase pretreatment and acid extraction of pectin from pomelo peels was performed in this study. By using a laccase dose of 10 U/g, the yield and galacturonic acid content of the extracted pectin were 21.67% and 75.74%, respectively, which reflected an improvement by nearly 30% and 44% relative to the extraction process without laccase. Moreover, the degree of esterification of the extracted pectin was significantly improved as the laccase concentration was increased from 0 to 10 U/g but did not affect the molecular weight. Besides, the enzyme-treated sample displayed more than 29% loss of total lignin, in which the acid soluble lignin and the acid insoluble lignin were removed by over 15% and 33%, respectively. Scanning electron microscopy indicated that the penetration and partial destruction of the pomelo peel cell wall due to laccase might be one of the main reasons why laccase pretreatment could release pectin from cell wall complexes and increase the pectin yield in subsequent acid extraction processes.

本研究采用漆酶预处理和酸提取柚皮果胶的顺序组合。使用10 U/g的漆酶剂量，提取果胶的得率和半乳糖醛酸含量分别为21.67%和75.74%，相对于不含漆酶的提取工艺提高了近30%和44%。此外，当漆酶浓度从 0 增加到 10 U/g 时，提取果胶的酯化程度显着提高，但不影响分子量。此外，酶处理的样品显示总木质素损失超过 29%，其中酸溶性木质素和酸不溶性木质素分别去除了 15% 和 33% 以上。