The co-culture of plant beneficial microbes to stimulate the production of antimicrobial metabolites is gaining ground. Here, the inactivated Colletotrichum gloeosporioides mycelium was used to induce the biosynthesis of antifungal compounds in the co-culture systems of Trichoderma sp. and Bacillus subtilis. The hexanic extracts obtained from the co-culture systems were tested against C. gloeosporioides. Those that inhibited the phytopathogen growth were further fractionated by column and thin-layer chromatography and analyzed by gas chromatography coupled to mass spectrometry (GC–MS). Ethyl butanoate, butyl acetate, acetic acid, 2-butoxyethanol, 3,5-di-tert-butyl-4-hydroxybenzaldehyde, 3,5-di-tert-butyl-4-hydroxybenzyl alcohol, hexadecanoic acid, and octadecanoic acid were identified. Butyl acetate was the most abundant compound, and its application affected the morphology and mycelial development of C. gloeosporioides, thereby inhibiting the radial growth, reducing spore formation, and inducing soft colonies. We conclude that co-culturing Trichoderma sp. and B. subtilis promotes the production of novel diffusible organic compounds with an antifungal effect on C. gloeosporioides.

植物有益微生物的共培养以刺激抗微生物代谢物的产生正在取得进展。在这里，灭活的炭疽菌菌丝体用于诱导木霉共培养系统中抗真菌化合物的生物合成。和枯草芽孢杆菌。对从共培养系统获得的己烷提取物进行了抗球孢子菌测试。那些抑制植物病原体生长的物质通过柱色谱和薄层色谱进一步分离，并通过气相色谱与质谱联用 (GC-MS) 进行分析。丁酸乙酯、乙酸丁酯、乙酸、2-丁氧基乙醇、3,5-二叔丁基-4-羟基苯甲醛、3,5-二叔丁基-4-羟基苯甲醇、十六烷酸和十八烷酸. 乙酸丁酯是含量最多的化合物，其应用影响了球孢子菌的形态和菌丝发育，从而抑制径向生长，减少孢子形成，诱导软菌落。我们得出结论，共培养木霉属。和枯草芽孢杆菌促进新的可扩散有机化合物的产生，对C. gloeosporioides具有抗真菌作用。