Restoration of fertility (Rf) is an important trait for pigeonpea hybrid breeding. Few coarse quantitative trait locus (QTL) studies conducted in the past identified QTLs with large confidence intervals on the genetic map and could not provide any information on possible genes responsible for Rf in pigeonpea. Therefore, a larger population comprising of 369 F2s derived from ICPA 2039 × ICPL 87119 was genotyped with high density Axiom Cajanus SNP Array with 56 K single nucleotide polymorphism (SNPs) for high resolution mapping of Rf. A genetic map with 4867 markers was developed and a total of four QTLs for Rf were identified. While one major effect QTL (qRf8.1) was co-localized with the QTL identified in two previous studies and its size was refined from 1.2 Mb to 0.41 Mb. Further analysis of qRf8.1 QTL with genome sequence provided 20 genes including two genes namely flowering locus protein T and 2-oxoglutarate/Fe (II)-dependent dioxygenases (2-ODDs) superfamily protein with known function in the restoration of fertility. The qRf8.1 QTL and the potential candidate genes present in this QTL will be valuable for genomics-assisted breeding and identification of causal genes/nucleotides for the restoration of fertility in the hybrid breeding program of pigeonpea.

中文翻译：

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木豆[Cajanus cajan（L.）Millsp]中的大量种群和高密度遗传图谱相结合的高分辨率恢复生育力（Rf）的图谱。

恢复生育力（Rf）是木豆杂交育种的重要特征。过去进行的粗略数量性状基因座（QTL）研究很少在遗传图谱上确定具有大置信区间的QTL，并且无法提供有关木豆中Rf的可能基因的任何信息。因此，使用具有56 K单核苷酸多态性（SNP）的高密度公理Cajanus SNP阵列对由ICPA 2039×ICPL 87119衍生的369个F2进行更大的基因分型，以进行Rf的高分辨率图谱分析。绘制了具有4867个标记的遗传图谱，总共鉴定出四个Rf的QTL。QTL（qRf8.1）是一个主要影响因素，与先前两项研究中确定的QTL共同定位，其大小从1.2 Mb精炼到0.41 Mb。qRf8的进一步分析。具有基因组序列的1个QTL提供了20个基因，包括开花基因蛋白T和2-氧戊二酸/ Fe（II）依赖性双加氧酶（2-ODDs）超家族蛋白这两个基因，它们具有恢复生育力的已知功能。qRf8.1 QTL和存在于该QTL中的潜在候选基因将对基因组学辅助育种和鉴定因果基因/核苷酸以恢复木豆杂种育种程序的育性具有重要意义。