Anti-osteosarcoma effect of hydroxyapatite nanoparticles both in vitro and in vivo by downregulating the FAK/PI3K/Akt signaling pathway.,Biomaterials Science

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Anti-osteosarcoma effect of hydroxyapatite nanoparticles both in vitro and in vivo by downregulating the FAK/PI3K/Akt signaling pathway.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-07-03 , DOI: 10.1039/d0bm00898b
Renxian Wang ₁ , WeiFeng Liu , Qian Wang , Guangping Li , Ben Wan , Yuyang Sun , Xiaohui Niu , Dafu Chen , Wei Tian

Affiliation

Numerous studies have reported that hydroxyapatite nanoparticles (nano-HAPs) can inhibit the proliferation of a variety of tumor cells and this effect is different in different carcinoma cells. However, the effect of nano-HAPs on osteosarcoma cell proliferation has not been well understood thus far. In this study, we first showed that our synthesized nano-HAPs reduced cell viability and inhibited migration and invasion of OS-732 cells in a concentration-dependent manner. Using a BALB/c nude mouse tumor model, we demonstrated that nano-HAPs could effectively suppress tumor growth in vivo. We also performed RNA-seq analysis to investigate the underlying mechanism of these effects and discovered that treatment of OS-732 cells with nano-HAPs significantly downregulated the FAK/PI3K/Akt signaling pathway. Collectively, our study suggests that treatment with nano-HAPs can inhibit osteosarcoma cell growth, migration and invasion in vitro and suppress osteosarcoma in vivo.

中文翻译：

通过下调FAK / PI3K / Akt信号通路在体外和体内羟基磷灰石纳米颗粒的抗骨肉瘤作用。

大量研究报告称，羟基磷灰石纳米颗粒（nano-HAPs）可以抑制多种肿瘤细胞的增殖，并且这种作用在不同的癌细胞中是不同的。然而，到目前为止，对纳米HAPs对骨肉瘤细胞增殖的作用还没有很好的了解。在这项研究中，我们首先表明我们合成的纳米HAP以浓度依赖的方式降低了细胞活力，并抑制了OS-732细胞的迁移和侵袭。使用BALB / c裸鼠肿瘤模型，我们证明了纳米HAP可以有效抑制体内肿瘤的生长。我们还进行了RNA序列分析以研究这些作用的潜在机制，并发现用nano-HAPs处理OS-732细胞可显着下调FAK / PI3K / Akt信号通路。总体而言，我们的研究表明，纳米HAPs的治疗可以在体外抑制骨肉瘤细胞的生长，迁移和侵袭，并在体内抑制骨肉瘤。

更新日期：2020-08-11

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