Wild blueberries rank very high in anthocyanins (ACNs) and phenolic acids (PA) among other berries. Previous work from our group has documented their beneficial effects. In this study, human umbilical vein endothelial cells were used, and ACNs, PAs, and their combination (ACNs:PAs) at concentrations of 0.002, 8, 15, 60, and 120 μg/mL were tested for endothelial tube formation. Treatment with ACNs decreased, while treatment with PAs and ACNs:PAs increased overall endothelial cell tube formation compared to control. Endothelial cells exposed to ACNs downregulated gene expression of AKT1 and endothelial nitric oxide synthase (eNOS), while PAs upregulated AKT1 and vascular endothelial growth factor (VEGF) gene expression. Combination of ACNs:PAs decreased gene expression of AKT1 and eNOS, while protein levels of AKT1 increased. In summary, based on the type of wild blueberry extract, angiogenesis is differentially modulated and is concentration dependent. Further experiments will delineate the mechanism(s) of the differential action of the aforementioned extracts on angiogenesis.

中文翻译：

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通过PI3K途径从野生蓝莓（V. angustifolium）中的花色苷和酚酸提取物差异调节血管生成。

野生蓝莓在其他浆果中的花色苷（ACN）和酚酸（PA）的含量很高。我们小组以前的工作已证明了它们的有益作用。在这项研究中，使用了人脐静脉内皮细胞，并测试了浓度为0.002、8、15、60和120μg/ mL的ACN，PA及其组合（ACN：PA）的内皮管形成情况。与对照组相比，使用ACN的治疗减少，而使用PA和ACNs：PA的治疗增加了总体内皮细胞管的形成。暴露于ACN的内皮细胞下调AKT1和内皮一氧化氮合酶（eNOS）的基因表达，而PA上调AKT1和血管内皮生长因子（VEGF）的基因表达。ACNs：PAs的结合降低了AKT1和eNOS的基因表达，而AKT1的蛋白质水平却升高了。总之，根据野生蓝莓提取物的类型，血管生成受到差异调节，并且与浓度有关。进一步的实验将描述上述提取物对血管生成的差异作用的机制。