Chemoproteomic Profiling of Cobalamin-Independent Methionine Synthases in Plants with a Covalent Probe.,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Chemoproteomic Profiling of Cobalamin-Independent Methionine Synthases in Plants with a Covalent Probe.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-07-03 , DOI: 10.1021/acs.jafc.0c03301
Xin Chen _{1,

2} , Jingyuan Xu ₁ , Nai-Kei Wong ₃ , Suyun Zhong ₁ , Mengquan Yang ₂ , Zhen Liu ₁ , Yan Lu ₁ , Weichao Li ₂ , Yiqing Zhou _{1,

2}

Affiliation

Cobalamin-independent methionine synthases (MS) are zinc-binding methyltransferases that catalyze de novo methionine biosynthesis in higher plants, which are enzymes critically involved in seed germination and plant growth. Here, we report a highly selective sulfonyl fluoride-based probe for chemoproteomic profiling of MS enzymes in living systems of the model plant Arabidopsis thaliana, as implemented in in-gel-, mass spectrometry-, and imaging-based platforms. This probe holds promise for facilitating and accelerating fundamental research and industrial application of MS enzymes, particularly in the contexts of MS1/2-targeting herbicide screening and design.

中文翻译：

具有共价探针的植物中钴胺素非依赖性蛋氨酸合酶的化学文谱分析。

不依赖钴胺素的蛋氨酸合酶（MS）是锌结合的甲基转移酶，可催化高等植物中的新生蛋氨酸生物合成，这是关键参与种子发芽和植物生长的酶。在这里，我们报告了一种高度选择性的基于磺酰氟的探针，用于在拟南芥模型植物的生命系统中进行MS酶的化学旋转谱分析，已在凝胶，质谱和成像平台中实现。该探针有望促进和加速MS酶的基础研究和工业应用，特别是在靶向MS1 / 2的除草剂筛选和设计的背景下。

更新日期：2020-07-29

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11