Arginase 1 (ARG1), typically expressed under stress or stimulation rather than in the healthy condition in humans. However, more and more studies claim ARG1 could be expressed by human and represents healing process. Including THP-1 monocyte shift to M2 macrophages rendered a higher expression of ARG1 than that with bone-graft has an ideal effect on anti-inflammation, resulting in tissue healing. With the higher expression of ARG1 seemingly induced by the constructed material and represented by the anti-inflammatory situation. For these reasons, this study focuses on whether traditional markers of macrophages could be express by human or not during clinical healing process. Through harvesting bone tissue samples during bone healing process and confirm the expression of markers at transcriptome and protein levels. The results showed the expression of ARG1 relatively low by bulk-RNA sequencing, quantitative polymerase chain reaction (qPCR), immunofluorescence (IF) and flow cytometry or could not be detected during process of healing of healthy donor. Taken together, results indicate that the ARG1 is not chosen for a regenerative marker in human bone tissue.

中文翻译：

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ARG1可以由人类表达，但不代表修复

精氨酸酶1（ARG1），通常在压力或刺激下表达，而不是在人类健康状态下表达。然而，越来越多的研究声称ARG1可以由人类表达并代表愈合过程。包括THP-1单核细胞向M2巨噬细胞的转移在内，ARG1的表达要高于骨移植对抗炎症的理想作用，从而导致组织愈合。似乎由所构建的材料诱导了ARG1的较高表达，并以抗炎状态为代表。由于这些原因，本研究集中在临床愈合过程中是否可以由人类表达传统的巨噬细胞标记物。通过在骨愈合过程中收集骨组织样品并确认转录组和蛋白质水平上标志物的表达。结果表明，通过体RNA测序，定量聚合酶链反应（qPCR），免疫荧光（IF）和流式细胞术检测ARG1的表达相对较低，或者在健康供体愈合过程中无法检测到。两者合计，结果表明未选择ARG1作为人骨组织中的再生标记。