Members of the Low Phosphate Root (LPR) family have been identified in rice (Oryza sativa) and expression analyses have been conducted. Here, we investigated the functions of one of the five members in rice, LPR5. qRT-PCR and promoter–GUS reporter analyses indicated that under Pi-sufficient conditions OsLPR5 was highly expressed in the roots, and specific expression occurred in the leaf collars and nodes, and its expression was increased under Pi-deficient conditions. In vitro analysis of the purified OsLPR5 protein showed that it exhibited ferroxidase activity. Overexpression of OsLPR5 triggered higher ferroxidase activity, and elevated concentrations of Fe(III) in the xylem sap and of total Fe in the roots and shoots. Transient expression of OsLPR5 in Nicotiana benthamiana provided evidence of its subcellular localization to the cell wall and endoplasmic reticulum. Knockout mutation in OsLPR5 by means of CRISPR-Cas9 resulted in adverse effects on Pi translocation, on the relative expression of Cis-NAT_OsPHO1;2, and on several morphological traits, including root development and yield potential. Our results indicate that ferroxidase-dependent OsLPR5 has both a broad-spectrum influence on growth and development in rice as well as affecting a subset of physiological and molecular traits that govern Pi homeostasis.

中文翻译：

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铁氧化酶LPR5在维持磷酸盐稳态中起作用，是水稻正常生长和发育所必需的。

该成员低磷根（LPR），家人都在水稻（已确定水稻）和表达分析已经进行。在这里，我们研究了水稻五种成员之一LPR5的功能。qRT-PCR和启动子– GUS报道者的分析表明，在Pi充足的条件下，OsLPR5在根中高表达，特异性表达在叶领和节中发生，在Pi不足的条件下其表达增加。纯化的OsLPR5蛋白的体外分析表明，它具有铁氧化酶活性。OsLPR5的过表达引发较高的铁氧化酶活性，木质部汁液中的Fe（III）浓度以及根和芽中的总Fe浓度升高。OsLPR5在本氏烟草中的瞬时表达提供了其亚细胞定位于细胞壁和内质网的证据。通过CRISPR-Cas9敲除OsLPR5突变对Pi转运，Cis-NAT _{OsPHO1; 2}的相对表达以及几种形态学特征（包括根发育和产量潜力）产生不利影响。我们的结果表明，依赖于铁氧化酶的OsLPR5 对水稻的生长和发育具有广泛的影响，并影响控制Pi动态平衡的一部分生理和分子性状。