Scientific Reports ( IF 4.6 ) Pub Date : 2020-07-03 , DOI: 10.1038/s41598-020-67745-5 Anna Dembska 1 , Angelika Świtalska 1 , Agnieszka Fedoruk-Wyszomirska 2 , Bernard Juskowiak 1
The properties of cytosine- and guanine-rich oligonucleotides contributed to employing them as sensing elements in various biosensors. In this paper, we report our current development of fluorescence oligonucleotide probes based on i-motif or G-quadruplex forming oligonucleotides for cellular measurements or bioimaging applications. Additionally, we also focus on the spectral properties of the new fluorescent silver nanoclusters based system (ChONC12-AgNCs) that is able to anchor at the Langmuir monolayer interface, which is mimicking the surface of living cells membrane.
中文翻译:
基于富含胞嘧啶和鸟嘌呤的序列的荧光寡核苷酸探针的开发。
富含胞嘧啶和鸟嘌呤的寡核苷酸的特性有助于将它们用作各种生物传感器中的传感元件。在本文中,我们报告了当前基于i-基序或G-四链体形成寡核苷酸的荧光寡核苷酸探针的最新进展,这些寡核苷酸探针可用于细胞测量或生物成像应用。此外,我们还将重点放在基于新型荧光银纳米簇的系统(ChONC12-AgNCs)的光谱特性上,该系统能够锚定在Langmuir单层界面上,该界面模仿了活细胞膜的表面。