The wide use of glyphosate-based herbicides (GBHs) has become a matter of concern due to its potential harmful effects on human health, including men fertility. This study sought to investigate, using the pig as a model, the impact of pure glyphosate and its most known commercial formulation, Roundup, on sperm function and survival. With this purpose, fresh commercial semen doses were incubated with different concentrations (0–360 µg/mL) of glyphosate (GLY; exp. 1) or Roundup, at the equivalent GLY concentration (exp. 2), at 38 °C for 3 h. Glyphosate at 360 µg/mL significantly (P < 0.05) decreased sperm motility, viability, mitochondrial activity and acrosome integrity but had no detrimental effect at lower doses. On the other hand, Roundup did significantly (P < 0.05) reduce sperm motility at ≥ 5 µg/mL GLY-equivalent concentration; mitochondrial activity at ≥ 25 µg/mL GLY-equivalent concentration; and sperm viability and acrosome integrity at ≥ 100 µg/mL GLY-equivalent concentration as early as 1 h of incubation. In a similar fashion, GLY and Roundup did not inflict any detrimental effect on sperm DNA integrity. Taken together, these data indicate that, while both glyphosate and Roundup exert a negative impact on male gametes, Roundup is more toxic than its main component, glyphosate.

草甘膦除草剂 (GBH) 的广泛使用已成为一个令人担忧的问题，因为它对人类健康（包括男性生育能力）具有潜在的有害影响。这项研究试图以猪为模型，研究纯草甘膦及其最著名的商业制剂农达对精子功能和存活的影响。为此，将新鲜商业精液与不同浓度 (0–360 µg/mL) 的草甘膦（GLY；实验 1）或农达（等效 GLY 浓度（实验 2））在 38 °C 下孵育 3 H。 360 µg/mL 的草甘膦显着降低精子活力、活力、线粒体活性和顶体完整性（ P < 0.05），但在较低剂量下没有有害影响。另一方面，当 GLY 当量浓度≥ 5 µg/mL 时，农达确实显着降低精子活力（ P < 0.05）；线粒体活性在 ≥ 25 µg/mL GLY 当量浓度下；早在孵化 1 小时，精子活力和顶体完整性就达到 ≥ 100 µg/mL GLY 当量浓度。以类似的方式，GLY 和农达不会对精子 DNA 完整性造成任何有害影响。总而言之，这些数据表明，虽然草甘膦和农达都对雄配子产生负面影响，但农达的毒性比其主要成分草甘膦更高。