The effect of the probiotic Shewanella putrefaciens Pdp11 (SpPdp11) was studied on the skin healing of experimentally wounded gilthead seabream (Sparus aurata L.). Two replicates (n = 12) of fish were fed CON diet or SP diet for 30 days. Half of the fish were sampled while the others were injured and sampled 7 days post-wounding. Results by image analysis of wound areas showed that SpPdp11 inclusion facilitated wound closure. Compared with the CON group, fish in SP group sampled 7 days post-wounding had a significantly decreased serum AST and increased ALB/GLOB ratio. Furthermore, protease and peroxidase activities were significantly increased in skin mucus from fish in SP group sampled 7 days post-wounding, compared with those fed CON diet. Additionally, SP diet up-regulated the gene expression of antioxidant enzymes, anti-inflammatory cytokines, and re-epithelialization related genes in the fish skin. Furthermore, significant decreases in pro-inflammatory cytokines expression were detected in fish from SP group, respect to control ones. Overall, SpPdp11 inclusion facilitated the wound healing and the re-epithelialization of the damaged skin, alleviated the inflammatory response in the wound area through intensifying the antioxidant system, and enhancing the neo-vascularization and the synthesis of matrix proteins in the skin wound sites of fish.

研究了益生菌腐烂希瓦氏菌Pdp11（SpPdp11）对实验受伤的金头鲷（Sparus aurata）皮肤愈合的影响L.）。将两份重复（n = 12）的鱼喂食CON饲料或SP饲料30天。一半的鱼在受伤后7天取样，其余的则受伤并取样。伤口区域的图像分析结果表明，SpPdp11包埋促进了伤口闭合。与CON组相比，SP组的鱼在伤后7天采样时血清AST显着降低，ALB / GLOB比升高。此外，与CON饮食相比，SP组在受伤后7天采样的鱼的皮肤粘液中蛋白酶和过氧化物酶活性显着增加。此外，SP饮食上调了鱼皮中抗氧化酶，抗炎细胞因子和上皮再生相关基因的基因表达。此外，与对照组相比，SP组鱼类的促炎细胞因子表达显着下降。总体而言，SpPdp11包合物促进了伤口的愈合和受损皮肤的重新上皮形成，通过增强抗氧化剂系统减轻了伤口区域的炎症反应，并增强了新生血管的形成和皮肤伤口部位基质蛋白的合成。鱼。