ABSTRACT

Early ripening is an important desirable attribute for fruit crops. ‘Tiangong Moyu’, a bud mutant of the grape cultivar ‘Summer Black’ (Vitis vinifera L.× Vitis labrusca L.), ripens nearly 10 days earlier and shows earlier increase in fresh weight, sugar accumulation, and color change and faster decrease in titratable acid than ‘Summer Black’. To identify the genes controlling early fruit development and ripening in ‘Tiangong Moyu’, RNA-Seq profiles of the two cultivars were compared at five different berry developmental stages. Based on GO annotation and KEGG pathway enrichment analysis, the differentially expressed genes were clustered into various functional groups, including plant hormone signal transduction, transcription factors, anthocyanin-associated biosynthesis pathway, phenylpropanoid biosynthesis, plant–pathogen associated and plant–pathogen interaction, and ripening-related protein genes. Our results provided a global transcriptional profile of candidate genes involved in regulating berry development and ripening and a genetic basis for the understanding of grape berry ripening.

摘要

早熟是水果作物的重要重要特性。“天工墨玉”，葡萄品种“夏黑”（的芽变葡萄大号。 ×美洲葡萄大号。），比“夏黑”早十天成熟，并且显示出鲜重，糖分积累和颜色变化以及可滴定酸的下降更快。为了确定控制“天宫漠鱼”早期果实发育和成熟的基因，在五个不同的浆果发育阶段比较了两个品种的RNA-Seq图谱。基于GO注释和KEGG途径富集分析，差异表达的基因被分为不同的功能组，包括植物激素信号转导，转录因子，花色苷相关的生物合成途径，苯丙烷类生物合成，植物-病原体相关和植物-病原体相互作用，以及成熟相关蛋白基因。