ABSTRACT

There is an increasing demand for horse milk, but the current domestic performance of the horse milk has not yet conducted a systematic in-depth study. We carried out a pilot study on 13 single nucleotide polymorphisms (SNPs) located within five genes in Kazakh horse using Kompetitive Allele Specific PCR (KASP^TM). An association study with average daily milk production of 60 Kazakh mares was performed for 6/13 polymorphic SNPs. The results showed that only two SNPs rs114262445 (NUMB), rs1136347938 (CA8) out of the 6 polymorphic markers were significantly associated with milk production in Kazakh horses. These results indicate the potential of using SNPs for the NUMB and CA8 genes as candidate genes for selection and farm production outputs. This study is relevant for future horse genomic studies.

摘要

对马乳的需求在增加，但是目前国内对马乳的性能尚未进行系统的深入研究。我们使用竞争性等位基因特异性PCR（KASP ^TM）对位于哈萨克马五个基因中的13个单核苷酸多态性（SNP）进行了初步研究。一项针对6/13多态性SNP进行的平均日产60头哈萨克母马的关联研究。结果显示，在6个多态性标记物中，只有两个SNP rs114262445（NUMB），rs1136347938（CA8）与哈萨克马的产奶量显着相关。这些结果表明在NUMB和CA8中使用SNP的潜力基因作为选择和农场生产输出的候选基因。这项研究与未来的马基因组研究有关。