Abstract

In present investigation carried out large-scale treatment of tannery effluent by the cultivation of microalgae, Neochloris aquatica RDS02. The tannery effluent treatment revealed that significant reduction heavy metals were chromium-3.59, lead-2.85, nickel-1.9, cadmium-10.68, zinc-4.49, copper-0.95 and cobalt-1.86 mg/L on 15th day of treatment using N. aquatica RDS02. The microalgal biosorption capacity q _max rate was Cr-88.66, Pb-75.87, Ni-87.61, Cd-60.44, Co-52.86, Zn-84.90 and Cu-54.39, and isotherm model emphasized that the higher R ² value 0.99 by Langmuir and Freundlich kinetics model. The microalga utilized highest CO₂ (90%) analyzed by CO₂ biofixation and utilization kinetics, biomass (3.9 mg/mL), lipid (210 mg mL⁻¹), carbohydrate (102.75 mg mL⁻¹), biodiesel (4.9 mL g⁻¹) and bioethanol (4.1 mL g⁻¹). The microalgal-lipid content was analyzed through Nile red staining. Gas chromatography mass spectrometric (GCMS) analysis confirmed that the presence of a biodiesel and major fatty acid methyl ester (FAME) profiling viz., tridecanoic acid methyl ester, pentadecanoic acid methyl ester, octadecanoic acid methyl ester, myristic acid methyl ester, palmitic acid methyl ester and oleic acid methyl ester. Fourier transform infrared (FTIR) analysis confirmed that the presence of a functional groups viz., phenols, alcohols, alkynes, carboxylic acids, ketones, carbonyl and ester groups. The bioethanol production was confirmed by high-performance liquid chromatography (HPLC) analyze.

摘要

在目前的研究中，通过培养微藻水生新藻（Neochloris aquatica RDS02）对制革厂废水进行了大规模处理。制革厂废水处理显示，使用水生北。草处理第15天，重金属的还原性显着还原为铬3.59，铅2.85，镍1.9，镉10.68，锌-4.49，铜0.95和钴1.86 mg / L。RDS02。微藻生物吸附容量q _最大率为铬88.66，铅75.87，镍- 87.61，CD-60.44，共52.86，锌84.90和Cu-54.39，和等温线模型强调的是，更高- [R ²由朗缪尔值0.99和Freundlich动力学模型。通过CO ₂分析，微藻利用了最高的CO ₂（90％）生物固定和利用动力学，生物量（3.9 mg / mL），脂质（210 mg mL ^-1），碳水化合物（102.75 mg mL ^-1），生物柴油（4.9 mL g ^-1）和生物乙醇（4.1 mL g ^-1）。通过尼罗红染色分析了微藻脂质的含量。气相色谱质谱（GCMS）分析确认存在生物柴油和主要脂肪酸甲酯（FAME），即十三烷酸甲酯，十五烷酸甲酯，十八烷酸甲酯，肉豆蔻酸甲酯，棕榈酸甲酯和油酸甲酯。傅立叶变换红外（FTIR）分析证实，官能团的存在即，酚，醇，炔烃，羧酸，酮，羰基和酯基。通过高效液相色谱（HPLC）分析确认了生物乙醇的产生。