ABSTRACT

Bone cements were prepared by mixing calcium sulfate and copper sulfate in various proportions. We examined physical and physicochemical properties of the copper doped calcium sulfates and the effects of the cements on angiogenesis in vivo. Rod shaped calcium sulfate crystals were visible by scanning electron microscopy in the cement that contained no copper sulfate, whereas plate-like crystals covered the surface of the cement with high copper content. After immersion of the cements in simulated body fluid (SBF) for 1 day, X-ray diffractometric analysis showed that gypsum precipitates had formed in the copper doped calcium sulfate. The compressive strength of the cements increased from 3.3 MPa for pure calcium sulfate to 6.4 MPa for samples with copper sulfate added. Calcium ion release was greatest from pure calcium sulfate, and the rate of copper ion release was higher for cements containing the most copper. We found that 6 weeks after implantation, more blood vessels had formed around the high copper cement than for the copper-free cement. Copper doped calcium sulfate appears to be useful for application to regenerative medicine including wound healing and bone tissue engineering.

摘要

通过将硫酸钙和硫酸铜以不同比例混合制备骨水泥。我们检查了铜掺杂硫酸钙的物理和物理化学特性以及水泥对体内血管生成的影响。通过扫描电子显微镜可以在不含硫酸铜的水泥中看到棒状硫酸钙晶体，而片状晶体覆盖在高铜含量的水泥表面。将水泥浸入模拟体液 (SBF) 1 天后，X 射线衍射分析表明，在掺铜硫酸钙中形成了石膏沉淀物。水泥的抗压强度从纯硫酸钙的 3.3 MPa 增加到添加硫酸铜的样品的 6.4 MPa。纯硫酸钙的钙离子释放量最大，含铜量最多的水泥，铜离子释放率较高。我们发现，植入后 6 周，高铜骨水泥周围形成的血管比无铜骨水泥多。掺铜硫酸钙似乎可用于再生医学，包括伤口愈合和骨组织工程。