TILRR has been identified as an important modulator of inflammatory responses. It is associated with NF-κB activation, and inflammation. Our previous study showed that TILRR significantly increased the expression of many innate immune responsive genes and increased the production of several pro-inflammatory cytokines/chemokines by cervical epithelial cells. In this study, we evaluated the effect of TILRR-induced pro-inflammatory cytokines/chemokines on the migration of immune cells. The effect of culture supernatants of TILRR-overexpressed cervical epithelial cells on the migration of THP-1 monocytes and MOLT-4 T-lymphocytes was evaluated using Transwell assay and a novel microfluidic device. We showed that the culture supernatants of TILRR-overexpressed HeLa cells attracted significantly more THP-1 cells (11–40%, p = 0.0004–0.0373) and MOLT-4 cells (14–17%, p = 0.0010–0.0225) than that of controls. The microfluidic device-recorded image analysis showed that significantly higher amount with longer mean cell migration distance of THP-1 (p < 0.0001–0.0180) and MOLT-4 (p < 0.0001–0.0025) cells was observed toward the supernatants of TILRR-overexpressed cervical epithelial cells compared to that of the controls. Thus, the cytokines/chemokines secreted by the TILRR-overexpressed cervical epithelial cells attracted immune cells, such as monocytes and T cells, and may potentially influence immune cell infiltration in tissues.

TILRR已被确定为炎症反应的重要调节剂。它与NF-κB活化和炎症有关。我们以前的研究表明，TILRR显着增加了许多先天免疫应答基因的表达，并增加了宫颈上皮细胞产生的几种促炎性细胞因子/趋化因子。在这项研究中，我们评估了TILRR诱导的促炎性细胞因子/趋化因子对免疫细胞迁移的影响。使用Transwell测定法和新型微流控装置评估了TILRR过度表达的宫颈上皮细胞培养上清液对THP-1单核细胞和MOLT-4 T淋巴细胞迁移的影响。我们发现，过表达TILRR的HeLa细胞的培养上清液吸引了更多的THP-1细胞（11–40％，p = 0.0004–0.0373）和MOLT-4电池（14–17％， p= 0.0010–0.0225）。微流控装置记录的图像分析显示，THP-1的平均细胞迁移距离更长，且数量明显更高（p <0.0001–0.0180）和MOLT-4（p与对照组相比，在TILRR过表达的子宫颈上皮细胞的上清液中观察到<0.0001–0.0025）的细胞。因此，由TILRR过表达的宫颈上皮细胞分泌的细胞因子/趋化因子吸引了免疫细胞，例如单核细胞和T细胞，并可能潜在地影响组织中免疫细胞的浸润。