Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance. Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials. The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications. In this contribution, we report a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels. Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3′-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds. Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability. In addition, the CCK-8 and live/dead assays demonstrated satisfying cytocompatibility of the CMC-OSA-DTP hydrogel in vitro. Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.

中文翻译：

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具有三重动态键的生物相容性、刺激响应性和可注射水凝胶


由于其侵入性最小的植入方法和易于操作的性能，可注射水凝胶作为有前途的临床应用生物材料引起了越来越多的兴趣。然而，基于天然生物材料的可注射水凝胶具有理想的无毒性，但其功能有限，无法满足临床生物材料的要求。开发兼具生物相容性和足够功能特性的新型可注射生物材料对于生物医学应用来说越来越紧迫。在这篇文章中，我们报告了一种简单有效的方法来制造多功能 CMC-OSA-DTP 水凝胶。利用两种天然多糖衍生的聚合物，羧甲基壳聚糖（CMC）和氧化海藻酸盐（OSA）以及3,3'-二硫代丙酸二酰肼（DTP）来引入三个动态共价键。由于三重动态键的存在，这种独特的CMC-OSA-DTP水凝胶具有智能氧化还原和pH刺激响应特性、可注射性以及自修复能力。此外，CCK-8 和活/死实验证明 CMC-OSA-DTP 水凝胶在体外具有令人满意的细胞相容性。基于其有吸引力的特性，这种易于制造的多功能水凝胶展示了作为可注射生物材料在各种生物医学应用中的巨大潜力。