Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. The quantitative detection of a low concentration of CEA has important medical significance. In this study, three-dimensional porous graphene-oxide-supported platinum metal nanoparticles (3DPt/HGO) composites were prepared by a wet chemical method and modified on an electrode with enhanced conductivity, a large surface area, and good adsorption of immobilizing antibodies (Ab₁). Horseradish peroxidase (HRP)-functionalized Au nanoparticles were fabricated to label the secondary antibodies (Ab₂). The proposed immunosensor showed a good linear relationship in the range of 0.001–150 ng/mL for CEA and a detection limit of 0.0006 ng/mL. The immunosensor had high sensitivity, good stability and reproducibility, and has great application prospects for the clinical diagnosis of cancer.

中文翻译：

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基于三维多孔纳米铂/石墨烯的肿瘤标记癌胚抗原（CEA）灵敏检测的电化学免疫传感器。

癌胚抗原（CEA）是重要的广谱肿瘤标志物。低浓度CEA的定量检测具有重要的医学意义。在这项研究中，通过湿化学方法制备了三维多孔氧化石墨烯负载的铂金属纳米颗粒（3DPt / HGO）复合材料，并在电极上进行了改性，以提高电导率，大表面积和固定抗体的良好吸附（ Ab ₁）。辣根过氧化物酶（HRP）-功能化的金纳米粒子被制造来标记第二抗体（Ab ₂）。所提出的免疫传感器对CEA表现出良好的线性关系，范围为0.001–150 ng / mL，检出限为0.0006 ng / mL。该免疫传感器灵敏度高，稳定性和重复性好，在癌症的临床诊断中具有广阔的应用前景。