The objective of this study was to explore the genetic and biological features of the tet(M)-harboring plasmid pTS14 in Salmonella enterica strain S14 isolated from a chicken fecal sample. Plasmid pTS14 was identified by conjugation, S1-pulsed-field gel electrophoresis (PFGE), Southern hybridization, and plasmid sequencing. The biological characteristics of pTS14 were assessed via stability, growth kinetics, and starvation survival experiments. Strain S14, belonging to ST3007, harbored a 119-kb tet(M)-bearing IncF2:A1:B1 conjugative plasmid pTS14. The plasmid pTS14 contained a novel transposon Tn6709 with the genetic structure IS26-tnpA1-tnpA2-Δorf13-LP-tet(M)-tnpX-ΔtnpR-IS26, and the resistance genes tet(B), tet(D), strAB, sul2, and blaTEM–1b. In addition, pTS14 was found to be highly stable in the recipient strain E. coli J53. The transconjugant TS14 exhibited a higher survival ratio than E. coli J53 under permanent starvation-induced stress. The tet(M)-bearing IncF2 epidemic plasmid lineage may accelerate the dissemination of tet(M) and other genes by coselection, which could constitute a potentially serious threat to clinical treatment regimens.
中文翻译:
pTS14的表征,一种在肠炎沙门氏菌中携带tet(M)的IncF2:A1:B1质粒。
这项研究的目的是探索该基因的遗传和生物学特征。 春节(M)携带质粒pTS14 肠沙门氏菌从鸡粪便样品中分离出的S14菌株。通过偶联,S1脉冲场凝胶电泳(PFGE),Southern杂交和质粒测序鉴定质粒pTS14。通过稳定性,生长动力学和饥饿存活实验评估了pTS14的生物学特性。属于ST3007的S14菌株含有119-kb春节(M)携带IncF2:A1:B1的结合质粒pTS14。质粒pTS14包含一个新的转座子Tn6709 具有遗传结构IS26--tnpA1--tnpA2-Δorf13--LP--春节(M)-tnpX-Δn-IS26和抗性基因 春节(B), 春节(D), strAB, sul2和 blaTEM–1b。另外,发现pTS14在受体菌株中高度稳定。大肠杆菌J53。转导结合体TS14的存活率高于大肠杆菌J53在永久饥饿诱导的压力下。的春节(M)携带IncF2流行质粒的谱系可能会促进 春节(M)和其他基因的共选,可能对临床治疗方案构成潜在的严重威胁。