Insulin is stored in secretory granules to facilitate rapid release in response to rising glucose levels, but the mechanisms by which these granules are identified and prioritized for secretion remains unclear. Using a fluorescent timer and flow cytometry–assisted organelle sorting, Yau et al. develop an elegant approach to assess insulin secretion as a function of granule age in pancreatic islet beta cells. Their findings supply quantitative evidence supporting the age-dependent release of different granule pools and confirm earlier models of preferential release of younger granules.

中文翻译：

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年龄很重要：β 细胞中颗粒分泌的分级。

胰岛素储存在分泌颗粒中，以便响应血糖水平升高而快速释放，但识别这些颗粒并优先分泌的机制仍不清楚。Yau 等人使用荧光计时器和流式细胞仪辅助细胞器分选。开发一种优雅的方法来评估胰岛素分泌作为胰岛β细胞颗粒年龄的函数。他们的研究结果提供了支持不同颗粒池的年龄依赖性释放的定量证据，并证实了优先释放年轻颗粒的早期模型。