Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China.,Molecular Ecology Resources

当前位置： X-MOL 学术 › Mol. Ecol. Resour. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China.
Molecular Ecology Resources ( IF 7.7 ) Pub Date : 2020-07-03 , DOI: 10.1111/1755-0998.13219
Lei Zhang ₁ , Bo Liu ₁ , Weigang Zheng ₁ , Conghui Liu ₁ , Dandan Zhang ₂ , Shengyuan Zhao ₂ , Zaiyuan Li ₁ , Pengjun Xu _{3,

4} , Kenneth Wilson _{1,

4} , Amy Withers ₄ , Christopher M Jones ₅ , Judith A Smith ₆ , Gilson Chipabika ₇ , Donald L Kachigamba ₈ , Kiwoong Nam ₉ , Emmanuelle d'Alençon ₉ , Bei Liu ₁ , Xinyue Liang ₁ , Minghui Jin ₁ , Chao Wu ₁ , Swapan Chakrabarty ₁ , Xianming Yang ₂ , Yuying Jiang ₁₀ , Jie Liu ₁₀ , Xiaolin Liu ₁₁ , Weipeng Quan ₁₂ , Guirong Wang ₁ , Wei Fan ₁ , Wanqiang Qian ₁ , Kongming Wu ₂ , Yutao Xiao ₁

Affiliation

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
Lancaster Environment Centre, Lancaster University, Lancaster, UK.
Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi.
School of Forensic and Applied Sciences, University of Central Lancashire, Preston, UK.
Zambia Agriculture Research Institute (ZARI), Lusaka, Zambia.
Department of Agricultural Research Services (DARS), Bvumbwe Research Station, Limbe, Malawi.
DGIMI, Univ. Montpellier, INRA, Montpellier, France.
National Agro-Tech Extension and Service Center, Beijing, China.
Novogene Bioinformatics Institute, Beijing, China.
Grandomics Biosciences, Co., Ltd, Beijing, China.

The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38‐Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22,260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bacillus thuringiensis (Bt) resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bt toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

更新日期：2020-07-03

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>