Bisphosphonates are prohibited drugs according to Article 6 of the International Agreement on Breeding, Racing and Wagering of the International Federation of Horseracing Authorities (IFHA) and the International Equestrian Federation (FEI). These compounds are used for the treatment of lameness, navicular and bone diseases in horses and are divided into two groups: non‐nitrogen‐containing bisphosphonate drugs (e.g. clodronic acid) and nitrogen‐containing bisphosphonate drugs (e.g. zoledronic acid). Their hydrophilic properties and the high affinity for the bone matrix make the control of their use quite difficult. Current analytical strategies to detect such compounds often rely on a solid phase extraction (SPE) followed by detection by means of UHPLC–MS/MS after methylation with chemical reagents. To improve the analysis throughput and to eliminate the need for chemical derivatization, an innovative 96‐well SPE followed by ion chromatography‐mass spectrometry was developed. Analyses are conducted on an ICS‐6000 HPIC system coupled to a TSQ Altis™ (Thermo Scientific™). The use of a 96‐well SPE allowed 5‐fold sample increase and a 6‐fold throughput improvement. While preliminary results conducted on horse plasma exhibited similar performances to the method for the detection of non‐nitrogen‐containing bisphosphonates, the analytical performances of nitrogen‐containing bisphosphonates were greatly improved.

中文翻译：

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一种创新的免衍生化 IC-MS/MS 方法，用于检测马血浆中的双膦酸盐。

根据国际赛马当局联合会 (IFHA) 和国际马术联合会 (FEI) 的《国际繁育、赛马和投注协议》第 6 条，双膦酸盐是禁用药物。这些化合物用于治疗马的跛行、舟骨和骨骼疾病，分为两类：不含氮的双膦酸盐药物（如氯膦酸）和含氮双膦酸盐药物（如唑来膦酸）。它们的亲水性和对骨基质的高亲和力使得控制它们的使用非常困难。目前检测此类化合物的分析策略通常依赖于固相萃取 (SPE)，然后在用化学试剂甲基化后通过 UHPLC-MS/MS 进行检测。为了提高分析通量并消除对化学衍生化的需要，开发了一种创新的 96 孔 SPE，然后进行离子色谱-质谱分析。在与 TSQ Altis™ (Thermo Scientific™) 耦合的 ICS-6000 HPIC 系统上进行分析。使用 96 孔 SPE 可使样品增加 5 倍，通量提高 6 倍。虽然对马血浆进行的初步结果显示出与检测非含氮双膦酸盐的方法相似的性能，但含氮双膦酸盐的分析性能得到了极大的提高。使用 96 孔 SPE 可使样品增加 5 倍，通量提高 6 倍。虽然对马血浆进行的初步结果显示出与检测非含氮双膦酸盐的方法相似的性能，但含氮双膦酸盐的分析性能得到了极大的提高。使用 96 孔 SPE 可使样品增加 5 倍，通量提高 6 倍。虽然对马血浆进行的初步结果显示出与检测非含氮双膦酸盐的方法相似的性能，但含氮双膦酸盐的分析性能得到了极大的提高。