This study aimed to evaluate the effect of miR‐10b on growth hormone (GH) in pituitary cells of Yanbian yellow cattle. According to analysis of GH and somatostatin receptor 2 (SSTR2) mRNA and protein expression levels, we found that miR‐10b targeted 3′UTR of SSTR2. Compared with the negative control (NC) group, GH mRNA transcription and protein expression in pituitary cells of Yanbian yellow cattle were significantly increased by adding miR‐10b mimics (p  < .01), while these were significantly decreased by adding miR‐10b inhibitor (p  < .05); compared with the NC group, SSTR2 mRNA transcription and protein expression were significantly inhibited by the addition of miR‐10b mimics (p  < .01), while these were significantly increased by the addition of miR‐10b inhibitor compared with the iNC group (p  < .05). This study suggested that miR‐10b could regulate GH level by regulating SSTR2 gene expression in pituitary cells of Yanbian yellow cattle.

中文翻译：

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miR-10b通过生长抑素受体2对延边黄牛垂体细胞生长激素的影响

本研究旨在评估miR-10b对延边黄牛垂体细胞生长激素（GH）的影响。根据对 GH 和生长抑素受体 2 (SSTR2) mRNA 和蛋白表达水平的分析，我们发现 miR-10b 靶向 SSTR2 的 3'UTR。与阴性对照 (NC) 组相比，添加 miR-10b 模拟物后延边黄牛垂体细胞 GH mRNA 转录和蛋白表达显着增加 ( p < .01)，而添加 miR-10b 抑制剂后显着 降低( p  < .05); 与 NC 组相比，添加 miR-10b 模拟物后，SSTR2 mRNA 转录和蛋白表达显着受到抑制（p < .01)，而与 iNC 组相比，添加 miR-10b 抑制剂后这些显着增加 ( p  < .05)。本研究表明miR-10b可通过调控延边黄牛垂体细胞SSTR2基因表达来调控GH水平。