Implementations of light‐sheet microscopes are often incompatible with standard methods of sample mounting. Light‐sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sample outside of the depth of field of the detection axis. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning and length of cover slips in the range of the detection objective. Here, we present an open‐hardware sample mounting system for light‐sheet microscopes using large detection objectives, built using 3D printed components and demonstrate the chamber's efficacy on live biological samples in a custom light‐sheet microscope.

中文翻译：

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用于具有大型检测物镜的倒置光片显微镜的开放硬件样品安装解决方案


光片显微镜的实施通常与样品安装的标准方法不兼容。光片显微镜使用正交照明和检测来产生薄薄的光片，该光片不会照亮检测轴景深之外的样品。通常，这种配置涉及一对正交物镜，其将盖玻片的位置和长度限制在检测物镜的范围内。在这里，我们提出了一种用于使用大型检测物镜的光片显微镜的开放硬件样品安装系统，该系统使用 3D 打印组件构建，并展示了腔室对定制光片显微镜中活体生物样品的功效。