Burbot (Lota lota maculosa ) are a potential new species for commercial aquaculture. As burbot culture expands, there is a need to further define pathogen susceptibility and characterize aspects of the burbot immune response in an effort to assess fish health. A recent clinical diagnostic case from juvenile burbot reared at a commercial production facility resulted in the isolation and identification of Flavobacterium columnare along with several Aeromonas spp. The F. columnare isolate was assigned to genetic group 1 via multiplex PCR, a genetic group commonly associated with columnaris disease cases in rainbow trout (Oncorhynchus mykiss ). Virulence of the F. columnare isolate was assessed in vivo in both juvenile burbot and rainbow trout. Additionally, several of the Aeromonas sp. case isolates were identified via sequencing (16S rRNA, gyrB and rpoD ) and a putative A . sobria isolate (BI‐3) was used to challenge burbot, along with a known virulent Aeromonas sp. (A141), but BI‐3 was not found to be virulent. Burbot were refractory to F. columnare when challenged by immersion, and it is likely that this is a secondary pathogen for burbot. Although refractory in burbot, the identified F. columnare isolate (BI‐1) was found to be virulent in rainbow trout.

中文翻译：

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用黄杆菌和气单胞菌对培养的江（Lota lota maculosa）的分离和实验挑战。隔离株。

Burbot（Lota lota maculosa）是商业水产养殖的潜在新物种。随着江鳕养殖的发展，有必要进一步定义病原体敏感性，并表征江堰免疫反应的各个方面，以评估鱼类的健康状况。最近在商业生产设施中饲养的幼年梭鱼的临床诊断病例导致分离和鉴定了黄杆菌和几种气单胞菌。所述F. columnare分离物通过多重PCR，通常与柱状病病例中虹鳟鱼（相关的遗传组分配到的遗传组1虹鳟）。柱形杆菌的毒力在幼年梭鱼和虹鳟鱼中对分离株进行了体内评估。此外，几个气单胞菌。通过测序（16S rRNA，gyrB和rpoD）和一个推定的A确定病例分离株。sobria分离物（BI-3）与已知的强毒气单胞菌sp。（A141），但未发现BI-3具有毒性。当受到浸没的挑战时，Burbot对F. columnare是难治的，这很可能是Burbot的次生病原体。尽管在Burbot中是难治性的，但已发现已鉴定的F. columnare分离株（BI-1）在虹鳟鱼中具有毒性。