Obesity is characterised by excessive accumulation of fat in white adipose tissue (WAT) which is compartmentalised into two anatomically and functionally diverse depots - visceral and subcutaneous. Advice to substitute essential polyunsaturated fatty acids (PUFAs) for saturated fatty acids is a cornerstone of various obesity management strategies. Despite an array of reports on the role of essential PUFAs on obesity, there still exists a lacuna on their mode of action in distinct depots i.e. visceral (VWAT) and subcutaneous (SWAT). The present study aimed to evaluate the effect of fish oil and corn oil on VWAT and SWAT in high-fat-diet-induced rodent model of obesity. Fish oil (FO) supplementation positively ameliorated the effects of HFD by regulating the anthropometrical and serum lipid parameters. FO led to an overall reduction in fat mass in both depots while specifically inducing beiging of adipocytes in SWAT as indicated by increased UCP1 and PGC1α. We also observed an upregulation of AMPKα and ACC1/2 phosphorylation on FO supplementation in SWAT suggesting a role of AMPK-PGC1α-UCP1 axis in beiging of adipose tissue. On the other hand, corn oil supplementation did not show any improvements in adipose tissue metabolism in both the depots of adipose tissue. The results were analysed using one-way ANOVA followed by Tukey's test in Graphpad Prism 5.0. Combined together our results suggest that n-3 PUFAs exert their anti-obesity effect by regulating adipokine secretion and inducing beiging of SWAT, hence increasing energy expenditure via thermogenic upregulation.

肥胖症的特征是脂肪在白色脂肪组织（WAT）中过度堆积，该脂肪被分隔为两个在解剖学和功能上各不相同的储藏室-内脏和皮下。用必需的多不饱和脂肪酸（PUFA）代替饱和脂肪酸的建议是各种肥胖管理策略的基石。尽管对肥胖必需PUFA的作用，报告一个数组，仍然存在明显的在油库它们的作用模式的一个漏洞，即内脏（VWAT）和皮下（SWAT）。本研究旨在评估鱼油和玉米油对高脂饮食诱导的肥胖鼠模型中VWAT和SWAT的影响。鱼油（FO）补充剂通过调节人体测量学和血清脂质参数，积极改善了HFD的作用。FO导致两个仓库的脂肪量总体减少，同时通过增加UCP1和PGC1α特异性地诱导SWAT中脂肪细胞的形成。我们还观察到SWAT中FO补充剂中AMPKα和ACC1 / 2磷酸化的上调，表明AMPK-PGC1α-UCP1轴在脂肪组织增白中的作用。另一方面，玉米油补充剂在两个脂肪组织的储库中均未显示出脂肪组织代谢的任何改善。使用单向方差分析，然后使用Tukey'分析结果 Graphpad Prism 5.0中的s测试。结合我们的研究结果表明，n-3 PUFA通过调节脂肪因子的分泌和诱导SWAT的产生来发挥其抗肥胖作用，从而增加了能量消耗通过生热上调。