Abstract Asiatic hybrid lily (Lilium spp.) cultivars ‘Centerfold’ and ‘Loreto’ developed an anthocyanin brushmark color pattern on the interior surfaces of flower tepals, which consisted of a set of streaks generated along the main vascular bundles. To clarify the mechanism underlying the brushmark pattern, R2R3-MYB transcription factors that positively regulated anthocyanin biosynthesis were screened in the two cultivars, and MYB19LONG (MYB19L) and MYB19SHORT (MYB19S) were identified. Among them, the expression profile of MYB19L correlated well with those of anthocyanin biosynthesis genes and pigment deposition in tepals. As MYB19L and MYB19S were recently identified as transcription factors regulating the pigmentation of raised spots on lily tepals, the correlation of MYB19L with brushmark and raised spot color patterns was evaluated using F1 populations by segregating the color patterns. MYB19L was always expressed in the F1 plants that developed brushmarks, indicating that MYB19L is necessary for brushmark pigmentation. The F1 plants that developed only raised spots expressed MYB19L, MYB19S, or both, suggesting that MYB19L is also involved in raised spot pigmentation. Segregation of the F1 populations showed that MYB19L was necessary but other gene(s) also participated in brushmark development. Finally, the roles of MYB19L in the development of brushmarks and raised spots are discussed.

中文翻译：

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MYB19LONG 参与亚洲杂交百合 (Lilium spp.) 花的刷痕模式开发

摘要 亚洲杂交百合 (Lilium spp.) 栽培品种 'Centerfold' 和 'Loreto' 在花被片的内表面形成了一种花青素刷痕颜色图案，由沿主维管束产生的一组条纹组成。为了阐明刷痕模式的潜在机制，在两个品种中筛选了正调控花青素生物合成的 R2R3-MYB 转录因子，并鉴定了 MYB19LONG (MYB19L) 和 MYB19SHORT (MYB19S)。其中，MYB19L 的表达谱与花青素生物合成基因和花被片中色素沉积的表达谱密切相关。由于 MYB19L 和 MYB19S 最近被鉴定为调节百合花被凸起斑点色素沉着的转录因子，MYB19L 与笔刷和凸起的专色图案的相关性使用 F1 种群通过分离颜色图案来评估。MYB19L 总是在产生刷痕的 F1 植物中表达，表明 MYB19L 是刷痕色素沉着所必需的。仅产生凸起斑点的 F1 植物表达 MYB19L、MYB19S 或两者，表明 MYB19L 也参与凸起斑点色素沉着。F1 种群的分离表明 MYB19L 是必要的，但其他基因也参与了刷状发展。最后，讨论了 MYB19L 在刷痕和凸起斑点发展中的作用。仅产生凸起斑点的 F1 植物表达 MYB19L、MYB19S 或两者，表明 MYB19L 也参与凸起斑点色素沉着。F1 种群的分离表明 MYB19L 是必要的，但其他基因也参与了刷状发展。最后，讨论了 MYB19L 在刷痕和凸起斑点发展中的作用。仅产生凸起斑点的 F1 植物表达 MYB19L、MYB19S 或两者，表明 MYB19L 也参与凸起斑点色素沉着。F1 种群的分离表明 MYB19L 是必要的，但其他基因也参与了刷状发展。最后，讨论了 MYB19L 在刷痕和凸起斑点发展中的作用。