JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review, we will present examples achieved using JSPR and demonstrate recently implemented features of JSPR such as multi-aberration "alignments" and automatic optimization of masking for the assessment of map resolution using "true" FSC.

JSPR 是普渡大学江实验室开发的单粒子冷冻电镜图像处理和 3D 重建软件。它最初是用于二十面体病毒的对称和不对称重建的一些改进脚本，但已经发展成为一套全面的工具，用于构建从头重建、病毒的高分辨率改进、任意对称的蛋白质复合物，包括螺旋管/细丝和图像文件处理实用程序。在这篇评论中，我们将展示使用 JSPR 实现的示例，并展示最近实现的 JSPR 功能，例如多像差“对齐”和使用“真实”FSC 评估地图分辨率的掩蔽自动优化。