Fluorescence quantum yield determination using the Simultaneous Absorption and Fluorescence Emission (SAFE) method can be performed on the spectrofluorometers which are equipped with the transmittance detector. However, the commercial spectrofluorometers are not commonly equipped with the transmittance detector, and those which possess this functionality are offered only in the single beam geometry. This seriously limits the application of the SAFE method. This article describes a simple approach to adapt a sample compartment of virtually any spectrofluorometer model for SAFE quantum yield determination. Furthermore, it shows the first application of a double-beam absorption measurement for spectrofluorometers. This approach provides the utmost signal stability, is inexpensive, and requires no hardware programming.

使用同时吸收和荧光发射（SAFE）方法的荧光量子产率测定可以在配备有透射率检测器的分光荧光计上进行。但是，商用分光荧光计通常不配备透射检测器，而具有此功能的仪器仅在单光束几何结构中提供。这严重限制了SAFE方法的应用。本文介绍了一种简单的方法，适用于几乎所有分光荧光计模型的样品室，用于SAFE量子产率测定。此外，它显示了用于分光荧光计的双光束吸收测量的首次应用。这种方法提供了最大的信号稳定性，价格便宜，并且不需要硬件编程。