Research on development of novel metal based anti-cancer agents continues with its popularity among bioinorganic community. Benzothiazole, an important heterocyclic pharmacophore, was chosen as a valuable and useful scaffold for the synthesis of novel copper(II) complexes. Three new copper(II) complexes obtained from the synthesis of newly synthesized benzothiazole based N-(benzo[d]thiazol-2-ylmethyl)-N-methyl-2-(pyridin-2-yl)ethan-1-amine (btzpy) ligand with CuCl₂ [Cu(btzpy)Cl₂] (1), Cu(NCS)₂ [Cu(btzpy)(NCS)₂] (2), and Cu(NO₃)₂ [Cu(btzpy)(NO₃)(H₂O)]NO₃ (3) were isolated and characterized by physical and spectroscopic measurements, including single-crystal X-ray structures. The interaction of complexes 1 and 3 with calf thymus (CT)-DNA was investigated using ethidium bromide fluorescence quenching assay and weak intercalation with K_SV values of 9.8 × 10² M⁻¹ and 8.2 × 10² M⁻¹, respectively was observed. All three complexes have shown DNA cleavage of supercoiled plasmid DNA forming single nicked and double nicked forms in the presence of external reducing agents like 3-mercaptopropionic acid (3-MPA) and ascorbic acid. The water-soluble complexes 1 and 3 also show prominent hydrolytic DNA cleavage. From the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, it was observed that complex 2 also exhibits good antioxidant properties. The cytotoxicity of complexes 1–3 was tested against the lung cancer cell line (A549) and complex 2 with -NCS moiety shows maximum activity in the micromolar range. A rationale for the observed activity is proposed in light of the other properties of these molecules.

随着新型金属基抗癌药在生物无机界的普及，其研究仍在继续。苯并噻唑是一种重要的杂环药效团，被选为合成新型铜（II）配合物的有价值和有用的支架。从新合成的基于苯并噻唑的N-（苯并[ d ]噻唑-2-基甲基）-N-甲基-2-（吡啶-2-基）乙-1-胺（btzpy）的合成中获得的三种新的铜（II）配合物）与CuCl ₂ [Cu（btzpy）Cl ₂ ]（1），Cu（NCS）₂ [Cu（btzpy）（NCS）₂ ]（2）和Cu（NO₃）₂ [Cu（btzpy）（NO ₃）（H ₂ O）] NO ₃（3）的分离和特征在于物理和光谱测量，包括单晶X射线结构。使用溴化乙锭荧光猝灭法和弱插层法研究了配合物1和3与小牛胸腺（CT）-DNA的相互作用，K _SV值为9.8×10 ²  M ^-1和8.2×10 ²  M ^-1，分别被观察到。在存在外部还原剂（例如3-巯基丙酸（3-MPA）和抗坏血酸）的情况下，所有三种复合物均显示超螺旋质粒DNA的DNA切割形成单切口和双切口形式。水溶性复合物1和3也显示出显着的水解DNA裂解。从DPPH（2，2-二苯基-1-吡啶并肼基）自由基清除测定中，观察到配合物2也表现出良好的抗氧化性能。复合物的细胞毒性1 - 3中针对肺癌细胞系（A549）和复杂的测试2具有-NCS部分的R 2在微摩尔范围内显示最大活性。根据这些分子的其他性质，提出了观察到的活性的基本原理。