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Purification and identification of anti-allergic peptide from Atlantic Salmon (Salmo salar) byproduct enzymatic hydrolysates
Journal of Functional Foods ( IF 3.8 ) Pub Date : 2020-07-03 , DOI: 10.1016/j.jff.2020.104084
Kexin Wang , Pramod Narayana Siddanakoppalu , Ishfaq Ahmed , Tushar Ramesh Pavase , Hong Lin , Zhenxing Li

Nature-derived anti-allergic peptides are considered one of the promising therapeutic approaches in alleviating food allergy. The present focus aims to open up a new high-value and full-use way for processing industrial fish wastes while expecting to obtain anti-allergic peptides. In this research, the anti-allergic peptide from Atlantic Salmon (Salmo salar) byproduct hydrolysate by pepsin hydrolysis, was purified and identified using consecutive chromatographic techniques such as Sephadex G-15 gel permeation chromatography, high performance liquid chromatography and LC/MS/MS. Among the fractions (C1-C6) obtained from RP-HPLC, C6 exerted the strongest anti-allergic activity (89.40%) significantly at 1 mg/mL (P<0.05). Afterwards, the novel peptide with eleven amino acids identified as TPEVHIAVDKF was proved to exert anti-allergic activity after synthesis by inhibiting the release of β-hexosaminidase in IgE-mediated RBL-2H3 cell degranulation at IC50 value: 1.39 mg/mL. Thus, Atlantic salmon byproduct can be a potential source of novel ingredients in food and pharmaceuticals for food allergy management.



中文翻译:

纯化和从大西洋鲑鱼抗过敏肽的识别(大西洋鲑)副产物酶水解产物

自然来源的抗过敏肽被认为是减轻食物过敏的有前途的治疗方法之一。本重点旨在开辟一种新的高价值和充分利用的方式来处理工业鱼废料,同时期望获得抗过敏肽。在这项研究中,通过胃蛋白酶水解纯化并鉴定了来自大西洋鲑鱼(Salmo salar)副产物水解物的抗过敏肽,并使用了Sephadex G-15凝胶渗透色谱,高效液相色谱和LC / MS / MS等连续色谱技术进行了鉴定。 。从RP-HPLC获得的馏分(C1-C6)中,C6在1 mg / mL时表现出最强的抗过敏活性(89.40%)(P<0.05)。之后,被鉴定为TPEVHIAVDKF的具有11个氨基酸的新型肽被证明在IC 50值为1.39 mg / mL时通过抑制IgE介导的RBL-2H3细胞脱粒过程中β-己糖胺酶的释放而具有抗过敏活性。因此,大西洋鲑鱼副产物可能是食品和药品中用于食品过敏管理的新型成分的潜在来源。

更新日期:2020-07-03
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