Our study was aimed at investigating the protective effect of low molecular weight peptides (SCHPs-F1) from red shrimp (Solenocera crassicornis) head against cyclophosphamide-induced hepatotoxicity in mice. We found that SCHPs-F1 treatment dose-dependently normalized the biochemical markers, hepatic index, and total CYP450 enzyme content in CTX-induced hepatotoxicity in mice. SCHPs-F1 also ameliorated the CTX-mediated structural disorders of the hepatic tissue. Western blot results suggested that SCHPs-F1 significantly restored the levels of endogenous antioxidants (CAT, T-AOC, GSH-Px, SOD and MDA levels) through activating the Nrf2 signal by upregulating the expression of GCLM, HO-1, and NQO-1. Moreover, SCHPs-F1 improved the CTX-induced hepatotoxicity by inhibiting of NF-κB signal responses and down-regulating the expression of the inflammatory factors (IL-1β, IL-6, IFN-γ and TNF-α). These findings suggest that SCHPs-F1 can regulate Nrf2 and NF-κB signaling pathways and reduce the oxidative stress and inflammation in CTX-induced hepatotoxicity. Overall, SCHPs-F1 are value-added food ingredients for alleviating CTX-induced hepatotoxicity.

我们的研究旨在调查红虾（Solenocera crassicornis）的低分子量肽（SCHPs-F1）的保护作用抵抗环磷酰胺诱导的小鼠肝毒性。我们发现SCHPs-F1治疗可以剂量依赖性地使CTX诱导的小鼠肝毒性中的生化标志物，肝指数和总CYP450酶含量正常化。SCHPs-F1还改善了CTX介导的肝组织结构异常。蛋白质印迹结果表明SCHPs-F1通过上调GCLM，HO-1和NQO-的表达来激活Nrf2信号，从而显着恢复内源性抗氧化剂的水平（CAT，T-AOC，GSH-Px，SOD和MDA含量）。 1。此外，SCHPs-F1通过抑制NF-κB信号反应并下调炎症因子（IL-1β，IL-6，IFN-γ和TNF-α）的表达来改善CTX诱导的肝毒性。这些发现表明SCHPs-F1可以调节Nrf2和NF-κB信号通路，并减少CTX诱导的肝毒性中的氧化应激和炎症。总体而言，SCHPs-F1是减轻CTX诱导的肝毒性的增值食品成分。