Glucose oxidase (GOx) is one of the most frequently used enzymes in a design of enzymatic biosensors and biofuel cells, which are novel electrical energy generation systems. Therefore, a better understanding of the mode of action of this enzyme is very important for further development of GOx-based sensors. In this research fluorescence properties of GOx in different acidic media have been estimated by the evaluation of redox states of active center that is flavine adenine dinucleotide (FAD). Steady-state fluorescence spectroscopy was applied to monitor the activity of GOx. A variation of pH has been invoked to gain a better understanding in the variations of GOx activity. The tendency of GOx activity to decrease over the time was determined, while increased intensity of the fluorescence band of GOx at 530 nm was associated with a decreased activity of the enzyme. The changes in fluorescence intensity of this band are caused by the dissociation of FAD from the enzyme. This process is not reversible, therefore, the decrease in the fluorescence intensity can be also associated with structural changes of the FAD during its reduction.

葡萄糖氧化酶（GOx）是酶促生物传感器和生物燃料电池（最新颖的电能产生系统）设计中最常用的酶之一。因此，更好地了解这种酶的作用方式对于进一步开发基于GOx的传感器非常重要。在这项研究中，通过评估活性中心黄酮腺嘌呤二核苷酸（FAD）的氧化还原状态，可以评估GOx在不同酸性介质中的荧光特性。应用稳态荧光光谱法监测GOx的活性。已调用pH的变化以获得对GOx活性变化的更好的理解。确定了GOx活性随时间下降的趋势，而在530 nm处GOx荧光带强度的增加与酶活性的降低有关。该条带的荧光强度变化是由于FAD从酶中解离而引起的。该过程是不可逆的，因此，荧光强度的降低也可能与FAD还原过程中的结构变化有关。