Structured lipids (SL) represent a new generation of lipids, considered bioactive compounds. Medium-chain, oleic (18:1n-9), and medium-chain fatty acid (MCFA) structured lipids (MOM-SL) were produced by acidolysis reaction in solvent-free medium with capric (10:0) and lauric (12:0) free fatty acids (FFAs) and triolein or olive oil, using Yarrowia lipolytica lipase as biocatalyst. MCFAs were rapidly incorporated into sn-1,3 SL in acidolysis reactions with triolein and olive oil, up until 30% of incorporation efficiency of capric and lauric acids in SLs. The kinetics of MCFA incorporation in MOM-SL was influenced by the FFA:TAG molar ratio, and for reactions between triolein and lauric acid, increasing FFA:TAG from 2:1 to 4:1 enhanced MCFA incorporation in SL. Y. lipolytica lipase showed a strictly 1,3-regioselective profile in acidolysis reaction, confirmed by nuclear magnetic resonance spectroscopy. Immobilization of this lipase by microencapsulation in chitosan-alginate beads resulted in similar incorporation efficiency for lauric acid with olive oil TAG and this reaction could be performed for 5 cycles without catalytic activity loss. This lipase showed promising properties as a potential biocatalyst that may be effectively used in production of bioactive structured lipids, which might be applied for prevention of metabolic and inflammatory disorders related to obesity.

结构化脂质（SL）代表了新一代脂质，被认为具有生物活性。中链，油酸（18：1n-9）和中链脂肪酸（MCFA）结构的脂质（MOM-SL）是在无溶剂的癸酸（10：0）和月桂酸（12）的无溶剂培养基中通过酸解反应制得的：0）使用解脂耶氏酵母脂肪酶作为生物催化剂的游离脂肪酸（FFA）和三油精或橄榄油。MCFA在与三油精和橄榄油的酸解反应中迅速掺入sn -1,3 SL中，直至癸酸中癸酸和月桂酸的掺入效率高达30％。MFA-SL中MCFA掺入的动力学受FFA：TAG摩尔比的影响，对于三油精和月桂酸之间的反应，将FFA：TAG从2：1增加到4：1可以增强MCFA在SL中的掺入。解脂耶氏酵母核磁共振波谱证实，脂肪酶在酸解反应中显示出严格的1,3-区域选择性。通过微囊化固定在壳聚糖-海藻酸盐珠粒中的这种脂肪酶导致月桂酸与橄榄油TAG的掺入效率相似，并且该反应可以进行5个循环而不会失去催化活性。该脂肪酶作为潜在的生物催化剂显示出有希望的性质，可以有效地用于生产生物活性结构脂质，该脂质可以用于预防与肥胖有关的代谢和炎性疾病。