Reconstruction of genome-based metabolic model is a useful approach for the assessment of metabolic pathways, genes and proteins involved in the environmental fitness capabilities or pathogenic potential as well as for biotechnological processes development. Pseudomonas sp. LFM046 was selected as a good polyhydroxyalkanoates (PHA) producer from carbohydrates and plant oils. Its complete genome sequence and metabolic model were obtained. Analysis revealed that the gnd gene, encoding 6-phosphogluconate dehydrogenase, is absent in Pseudomonas sp. LFM046 genome. In order to improve the knowledge about LFM046 metabolism, the coenzyme specificities of different enzymes was evaluated. Furthermore, the heterologous expression of gnd genes from Pseudomonas putida KT2440 (NAD⁺ dependent) and Escherichia coli MG1655 (NADP⁺ dependent) in LFM046 was carried out and provoke a delay on cell growth and a reduction in PHA yield, respectively. The results indicate that the adjustment in cyclic Entner-Doudoroff pathway may be an interesting strategy for it and other bacteria to simultaneously meet divergent cell needs during cultivation phases of growth and PHA production.

基于基因组的代谢模型的重建是评估与环境适应能力或致病潜力有关的代谢途径，基因和蛋白质以及生物技术过程发展的有用方法。假单胞菌属。LFM046被选作碳水化合物和植物油中的优良聚羟基链烷酸酯（PHA）生产商。获得了其完整的基因组序列和代谢模型。分析表明，假单胞菌属种中不存在编码6-磷酸葡糖酸脱氢酶的gnd基因。LFM046基因组。为了提高对LFM046代谢的了解，评估了不同酶的辅酶特异性。此外，gnd的异源表达分别从LFM046中获得了恶臭假单胞菌KT2440（NAD ⁺依赖性）和大肠杆菌MG1655（NADP ⁺依赖性）的基因，分别引起细胞生长延迟和PHA产量降低。结果表明，循环Entner-Doudoroff途径的调节可能是它和其他细菌在生长和PHA生产的培养阶段同时满足不同细胞需求的有趣策略。