Abstract The microbial community composition in subsurface oil reservoirs is responsible for the biogeochemical reactions of hydrocarbon transformation. In this study, the composition, major functions and gene expression activity of the microorganisms in production water from an oil reservoir were analyzed prior to and after injection nutrients using high-throughput 16S rRNA gene sequencing, functional gene and their transcript profiling. Pseudomonas spp. was the dominant bacteria showing a quick increase in the bacterial population. The injection of nutrients lowered the activity of assA-bearing bacteria, but accelerated the activity of methanogens. The results show that the anaerobic hydrocarbon degradation was actively occurring in situ oil reservoir sampled, Smithella was the active anaerobic hydrocarbon degrading bacteria, and Methanoculleus and Methanothrix were the actively dominant methanogens. The present study provides a snapshot of the practical technology with insightful results to support the research, development and utilization of reservoir through biological augmentation.

中文翻译：

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功能基因及其转录分析揭示了假单胞菌在细菌群落中的优势和通过向油藏注入营养物抑制延胡索酸添加途径

摘要 地下油藏微生物群落组成是烃类转化生物地球化学反应的关键。在这项研究中，使用高通量 16S rRNA 基因测序、功能基因及其转录谱分析了在注入营养物之前和之后油藏生产水中微生物的组成、主要功能和基因表达活性。假单胞菌属 是优势细菌，显示细菌数量迅速增加。营养物的注入降低了携带 assA 的细菌的活性，但加速了产甲烷菌的活性。结果表明，在取样的原位油藏中，厌氧烃降解活跃，Smithella 是厌氧烃降解活性菌，Methanoculleus 和 Methanothrix 是活跃的优势产甲烷菌。本研究提供了实用技术的快照，并提供了富有洞察力的结果，以通过生物增强来支持油藏的研究、开发和利用。