Abstract It is critical to maintain low levels of microbes in the whole food production chain. Due to high speed of slaughter, lack of time, and structural characteristics of crates, sufficient cleaning and disinfection of crates used for transporting chickens to abattoirs is a challenge. Inadequately cleaned transport crates for broiler chickens caused a major outbreak of campylobacteriosis in Sweden in 2016–2017, when the contaminated crates introduced Campylobacter to the chickens during thinning. This study evaluated the antibacterial efficacy of 265-nm ultraviolet (UV–C) LED light on artificially contaminated chicken transport crates. In a laboratory study, a transport crate artificially contaminated with Campylobacter and cecum contents was irradiated with 265-nm UV-C light by a continuous LED array in a treatment cabinet. The transport crate was sampled 52 times by cotton swabs before and after UV-C treatment for 1 min (20.4 mJ/cm2) and 3 min (61.2 mJ/cm2). The swab samples were analysed for Campylobacter jejuni (C. jejuni), bacteria belonging to the family Enterobacteriaceae, and total aerobic bacteria. After irradiation with UV-C LED light for 1 min, a mean reduction in C. jejuni of log 2.0 ± 0.5 CFU/mL was observed, while after irradiation for 3 min the reduction was log 3.1 ± 1.0 CFU/mL. The mean reduction in Enterobacteriaceae was log 1.5 ± 0.3 CFU/mL after 1 min of irradiation and log 1.8 ± 0.8 CFU/mL after 3 min. The mean reduction in total aerobic bacteria was log 1.4 ± 0.4 CFU/mL after 1 min of irradiation and log 1.6 ± 0.5 CFU/mL after 3 min. Significant reductions in bacterial load were observed in all samples after UV-C treatment and extending the treatment time from 1 to 3 min significantly increased the reduction in C. jejuni. However, before implementation of UV-C LED treatment in commercial chicken abattoirs, the irradiation unit would need to be extended and/or the washing procedure before UV-C treatment, to reduce the amount of organic matter on transport crates, would need to be improved.

中文翻译：

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通过 265 nm 紫外光 (UV-C LED) 照射减少鸡运输箱上的空肠弯曲杆菌、肠杆菌科和总需氧菌

摘要 在整个食品生产链中保持低水平的微生物至关重要。由于屠宰速度快、时间紧迫，以及板条箱的结构特点，用于将鸡运送到屠宰场的板条箱的充分清洁和消毒是一个挑战。2016-2017 年，未充分清洁的肉鸡运输箱导致瑞典发生弯曲杆菌病的大爆发，当时受污染的板条箱在变薄期间将弯曲杆菌引入了鸡。本研究评估了 265 nm 紫外线 (UV-C) LED 灯对人工污染的鸡运输箱的抗菌功效。在实验室研究中，通过处理柜中的连续 LED 阵列用 265 纳米 UV-C 光照射人工污染了弯曲杆菌和盲肠内容物的运输箱。在 UV-C 处理 1 分钟（20.4 mJ/cm2）和 3 分钟（61.2 mJ/cm2）之前和之后，用棉签对运输箱进行了 52 次采样。对拭子样本进行了空肠弯曲杆菌 (C. jejuni)、肠杆菌科细菌和总需氧细菌分析。用 UV-C LED 光照射 1 分钟后，观察到空肠弯曲杆菌的平均减少 log 2.0 ± 0.5 CFU/mL，而照射 3 分钟后，减少为 log 3.1 ± 1.0 CFU/mL。肠杆菌科细菌的平均减少量在照射 1 分钟后为 log 1.5 ± 0.3 CFU/mL，在 3 分钟后为 log 1.8 ± 0.8 CFU/mL。辐照 1 分钟后总需氧菌的平均减少为 log 1.4 ± 0.4 CFU/mL，3 分钟后为 log 1.6 ± 0.5 CFU/mL。在 UV-C 处理后，在所有样品中观察到细菌负荷显着减少，将处理时间从 1 分钟延长到 3 分钟，显着增加了空肠弯曲杆菌的减少。然而，在商业鸡屠宰场实施 UV-C LED 处理之前，需要延长辐照单元和/或 UV-C 处理前的清洗程序，以减少运输板条箱上的有机物数量，需要改进。