Enzymatic transformation of xylans into renewable fuels and value-added products is mediated by xylanases. Here we describe the biochemical and X-ray structural characterization of Thermobacillus composti GH10 xylanase (TcXyn10A) at 2.1 Å resolution aiming to unravel details of its recognition of glucurono- and arabinoxylan at a molecular level. TcXyn10A improves the efficiency of pretreated lignocellulosic biomass hydrolysis by a commercial enzyme cocktail causing a 15.35 % increase in xylose release and 4.38 % glucose release after 24 h of reaction. The enzyme releases predominantly xylobiose and xylotriose, as well as MeGlcA3 × 3 (from beechwood glucuronoxylan) and a range of decorated xylooligosaccharides (XOS) from rye arabinoxylan, with Ara2 × 2 being the major product. The enzyme liberates XOS with the yields of 29.09 % for beechwood glucuronoxylan and 16.98 % for rye arabinoxylan. Finally, TcXyn10A has a high thermal stability, halotolerance, and resistance to ethanol, biochemical properties that can be desirable for a number of industrial applications.

木聚糖酶转化为可再生燃料和增值产品的过程是由木聚糖酶介导的。在这里，我们以2.1Å分辨率描述了堆肥嗜热芽孢杆菌GH10木聚糖酶（Tc Xyn10A）的生化和X射线结构表征，旨在揭示其在分子水平上对葡萄糖醛酸和阿拉伯木聚糖的识别细节。cXyn10A通过商业酶混合物提高了预处理木质纤维素生物质水解的效率，导致反应24小时后木糖释放增加15.35％，葡萄糖释放增加4.38％。该酶主要释放木糖和木三糖，以及MeGlcA3×3（来自山毛榉葡糖醛酸木聚糖）和一系列修饰的木寡糖（XOS），来自黑麦阿拉伯木聚糖，主要成分是Ara2×2。该酶释放出XOS，对山毛榉木葡糖醛酸木聚糖的产率为29.09％，对于黑麦阿拉伯木聚糖的产率为16.98％。最后，Tc Xyn10A具有很高的热稳定性，耐盐性和抗乙醇性，这是许多工业应用所希望的生化特性。